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    Article

    Development of SNP markers linked to purple blotch resistance for marker-assisted selection in onion (Allium cepa L.) breeding

    Purple blotch (PB), caused by Alternaria porri (Ellis) Cifferi, is one of the most destructive diseases of onion worldwide. Rapid development and deployment of resistant onion varieties is the most effective appr...

    Jayashree Sahoo, Bijayalaxmi Mahanty, Rukmini Mishra, Raj Kumar Joshi in 3 Biotech (2023)

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    Chapter

    Targeted Gene Replacement in Plants Using CRISPR-Cas Technology

    The CRISPR-Cas based genome editing systems have been widely used in plant functional genomics research and crop improvement due to its simplicity, high efficiency and specificity. For example, the CRISPR-Cas9...

    Zheng Wei, Rukmini Mishra, Raj Kumar Joshi in Genome Editing Technologies for Crop Impro… (2022)

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    Chapter

    MicroRNA-Mediated Insect Resistance in Field Crops

    MicroRNA (miRNA)-mediated regulation of cell growth and development, gene translational repression, and stress responses have been extensively evaluated over the last two decades. Recent evidences indicate tha...

    Bijayalaxmi Mahanty, Suhas Sutar Bharat in Molecular Advances in Insect Resistance of… (2022)

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    Chapter

    Expanding the Scope of Base Editing in Crops Using Cas9 Variants

    Genome editing technologies hold tremendous possibilities towards crop improvement and food security for the future. Among others, base editors have developed as novel editing platforms that facilitates specif...

    Rukmini Mishra, Muntazir Mushtaq in Genome Editing Technologies for Crop Impro… (2022)

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    Chapter

    Genome Editing Is Revolutionizing Crop Improvement

    The ever-increasing human population together with emerging environmental cues from climate change demand for novel innovations in plant breeding and agriculture. Targeted genome editing technologies especiall...

    Rukmini Mishra, Raj Kumar Joshi, Kaijun Zhao in Genome Editing Technologies for Crop Impro… (2022)

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    Chapter

    Genome Editing Toward Rice Improvement

    Rice (Oryza sativa L.) serves as the major food source feeding half of the world’s population. In the past century, the pedigree-based conventional, radiation-based mutational and DNA marker-based molecular breed...

    Kaijun Zhao, Rukmini Mishra, Raj Kumar Joshi in Genome Editing Technologies for Crop Impro… (2022)

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    Article

    A single transcript CRISPR/Cas9 mediated mutagenesis of CaERF28 confers anthracnose resistance in chilli pepper (Capsicum annuum L.)

    T-DNA-free homozygous mutant lines developed through a single transcript CRISPR/Cas9 system harboring the desired modification in the CaERF28 locus exhibited significantly enhanced resistance to the anthracnose p...

    Rukmini Mishra, Jatindra Nath Mohanty, Bijayalaxmi Mahanty, Raj Kumar Joshi in Planta (2021)

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    Article

    Genome wide identification and expression analysis of pepper C2H2 zinc finger transcription factors in response to anthracnose pathogen Colletotrichum truncatum

    Although, the C2H2 zinc finger (ZF) family of plant transcription factors have been implicated in multiple biological processes, they are yet to be characterized in the economically important chilli pepper (Capsi...

    Richa Sharma, Bijayalaxmi Mahanty, Rukmini Mishra, Raj Kumar Joshi in 3 Biotech (2021)

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    Article

    Engineering drought tolerance in plants through CRISPR/Cas genome editing

    Drought stress is primarily responsible for heavy yield losses and productivity in major crops and possesses the greatest threat to the global food security. While conventional and molecular breeding approache...

    Raj Kumar Joshi, Suhas Sutar Bharat, Rukmini Mishra in 3 Biotech (2020)

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    Article

    Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)

    Anthracnose, caused by Colletotrichum spp. is the most devastating disease of chili (Capsicum annuum) in the tropical and subtropical regions of the world. The present study aimed at molecular map** and develop...

    Rukmini Mishra, Ellojita Rout, Jatindra Nath Mohanty, Raj Kumar Joshi in 3 Biotech (2019)

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    Article

    Genome editing technologies and their applications in crop improvement

    Crop improvement is very essential to meet the increasing global food demands and enhance food nutrition. Conventional crop-breeding methods have certain limitations such as taking lot of time and resources, a...

    Rukmini Mishra, Kaijun Zhao in Plant Biotechnology Reports (2018)