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Article
Open AccessCDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma
In oral squamous cell carcinoma (OSCC), a highly aggressive and frequently lethal malignancy, the role and action mechanism of the microtubule regulatory protein CDK5RAP2 have not been fully understood. Here, ...
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Protocol
Detection and Analysis of Microtubule Nucleator γ-Tubulin Ring Complex
Golgi-derived microtubules constitute an asymmetrical microtubule network that drives polarized transport of vesicles to support cell polarization and directional migration. Golgi-based microtubule nucleation ...
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Article
Epigenetic Alterations of Wnt Signaling Pathways in Nasopharyngeal Carcinoma
The purpose of this study is to discuss the epigenetic alterations of Wnt signaling pathways in nasopharyngeal carcinoma.
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Article
Open AccessThe catalytic subunit of DNA polymerase δ inhibits γTuRC activity and regulates Golgi-derived microtubules
γ-Tubulin ring complexes (γTuRCs) initiate microtubule growth and mediate microtubule attachment at microtubule-organizing centers, such as centrosomes and the Golgi complex. However, the mechanisms that contr...
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Chapter
Bioinformatic Analysis of Data Generated from MALDI Mass Spectrometry for Biomarker Discovery
In this chapter we first describe the applications of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) in biomarker discovery. After a summary of the general analysis pipeline of MALD...
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Article
The neuronal p35 activator of Cdk5 is a novel F-actin binding and bundling protein
The neuronal Cdk5 activator p35 is involved in a multitude of neuronal activities, including cytoskeletal organization. We show here that p35 directly interacts with filamentous actin (F-actin) but not with mo...
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Chapter and Conference Paper
Optimization-Based Peptide Mass Fingerprinting for Protein Mixture Identification
In current proteome research, the most widely used method for protein mixture identification is probably peptide sequencing. Peptide sequencing is based on tandem Mass Spectrometry (MS/MS) data. The disadvanta...
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Chapter
Protein–Protein Interactions Involving the N-Terminus of p35
As a major regulator of Cdk5, the protein p35 exerts various effects on neuronal cells. Under certain conditions, p35 undergoes truncation of its N-terminus to form the protein p25. Although p25 is equally pot...
