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Article
Open AccessAuthor Correction: Bases of Bacterial Sodium Channel Selectivity Among Organic Cations
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessBases of Bacterial Sodium Channel Selectivity Among Organic Cations
Hille’s (1971) seminal study of organic cation selectivity of eukaryotic voltage-gated sodium channels showed a sharp size cut-off for ion permeation, such that no ion possessing a methyl group was permeant. U...
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Article
Inhibition of a cardiac sarcoplasmic reticulum chloride channel by tamoxifen
Anion and cation channels present in the sarcoplasmic reticulum (SR) are believed to be necessary to maintain the electroneutrality of SR membrane during Ca2+ uptake by the SR Ca2+ pump (SERCA). Here we incorpora...
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Article
High Sensitivity, Quantitative Measurements of Polyphosphate Using a New DAPI-Based Approach
Polyphosphate (poly-P) is an important metabolite and signaling molecule in prokaryotes and eukaryotes. DAPI (4′,6-diamidino-2-phenylindole), a widely used fluorescent label for DNA, also interacts with polyph...
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Article
Involvement of a novel C-terminal kinase domain of Kir6.2 in the K-ATP channel rundown reactivation
Rundown is a generally encountered problem while recording KATP channel activity with inside-out patches. No assigned structural fragment related to this mechanism has yet been derived from any of the functiona...
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Chapter
Shifts of Macroscopic Current Activation in Partially Blocked Sodium Channels. Interaction between the Voltage Sensor and a μ-Conotoxin
The μ-conotoxins (μCTXs) are peptide toxins from carnivorous marine snails of the genus Conus, that specifically block voltage-dependent sodium channels from adult skeletal muscle (Moczydlowski et al., 1986; C...
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Protocol
Recording and Analysis of Currents from Single Ion Channels
In this chapter, we will give an overview of the methods for making electrical recordings from single ion channels in cell membranes and in planar lipid bilayers, endeavoring to point out the advantages and li...
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Chapter
Gating of Batrachotoxin-Activated Sodium Channels in Lipid Bilayers
Sodium channels incorporated into planar lipid bilayers share many fundamental properties with sodium channels in general and thus provide a useful system for the study of various aspects of channel behavior. ...
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Chapter
From Brain to Bilayer
Contemporary studies on the molecular basis of membrane excitability spring from the pivotal conclusion reached by Hodgkin and Huxley (1952b) that the currents that generate an action potential must flow throu...
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Article
Voltage-dependent calcium channels from brain incorporated into planar lipid bilayers
Many important physiological processes, including neurotransmitter release and muscle contraction1–3, are regulated by the concentration of Ca2+ ions in the cell. Levels of cytoplasmic Ca2+ can be elevated by the...
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Article
Single sodium channels from rat brain incorporated into planar lipid bilayer membranes
A voltage- and time-dependent conductance for sodium ions is responsible for the generation of impulses in most nerve and muscle cells1. Changes in the sodium conductance are produced by the opening and closing o...
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Chapter and Conference Paper
Finite Difference Methods for the Numerical Solution of the Nernst-Planck-Poisson Equations
In this paper I shall describe two methods for the numerical solution of the kinetic equations for electrolyte diffusion and suggest conditions under which these methods might be applied to advantage in studie...
