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  1. No Access

    Protocol

    The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions

    Adhesive interactions of cells with blood vessel walls under flow conditions are critical to a variety of processes, including hemostasis, leukocyte trafficking, tumor metastasis, and atherosclerosis. We have ...

    Robert Sackstein, Robert Fuhlbrigge in Cell-Cell Interactions (2006)

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    Protocol

    Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay

    Western blotting has proven to be an important technique in analysis of receptor-ligand interactions (i.e., by ligand blotting) and for identifying molecules mediating cell attachment (i.e., by cell blotting)....

    Robert Sackstein, Robert Fuhlbrigge in Protein Blotting and Detection (2009)

  3. No Access

    Article

    Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism

    FABP4 and FABP5 are important for the maintenance, longevity and function of CD8+ tissue-resident memory T cells, which use oxidative metabolism of exogenous free fatty acids to persist in tissues and to mediate ...

    Youdong Pan, Tian Tian, Chang Ook Park, Serena Y. Lofftus, Shenglin Mei, **ng Liu… in Nature (2017)