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Protocol
Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis
The giant embryo of the scarlet runner bean (Phaseolus coccineus) has been used historically to investigate the molecular and developmental processes that control the early events of plant embryo development. In ...
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Article
Open AccessA cis-regulatory module activating transcription in the suspensor contains five cis-regulatory elements
Little is known about the molecular mechanisms by which the embryo proper and suspensor of plant embryos activate specific gene sets shortly after fertilization. We analyzed the upstream region of the Scarlet ...
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Article
Open AccessEffects of APETALA2 on embryo, endosperm, and seed coat development determine seed size in Arabidopsis
Arabidopsis APETALA2 (AP2) controls seed mass maternally, with ap2 mutants producing larger seeds than wild type. Here, we show that AP2 influences development of the three major seed compartments: embryo, endosp...
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Article
Differentiation and degeneration of cells that play a major role in tobacco anther dehiscence
Dehiscence is the terminal step in anther development that releases pollen grains from the wall of each theca at a specific site between the two locules. In tobacco, two groups of cells—the circular cell clust...
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Article
fist: an Arabidopsis mutant with altered cell division planes and radial pattern disruption during embryogenesis
A T-DNA-tagged, embryo-defective Arabidopsis thaliana mutant, fist, was identified and shown to exhibit defects in nuclear positioning and cell division orientation beginning at the four-cell stage of the embryo...
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Chapter
Embryogenesis in Dicotyledonous Plants
Embryogenesis in higher plants establishes the basic shoot-root body pattern, the primary tissue layers, and the meristematic zones of the plant. Continuous differentiation of the meristems is the basis of pos...
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Article
A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants
Male fertility was restored to genetically engineered male sterile oilseed rape plants. Male sterile plants that express a chimaeric ribonuclease gene in the anther tapetal cell layer were crossed with male fe...
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Article
Induction of male sterility in plants by a chimaeric ribonuclease gene
Chimaeric ribonuclease genes that are expressed in the anthers of transformed tobacco and oilseed rape plants were constructed. Chimaeric ribonuclease gene expression within the anther selectively destroys the...
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Article
Interaction of an embryo DNA binding protein with a soybean lectin gene upstream region
Seed protein genes are highly regulated during the soybean life cycle1,2. These genes encode prevalent mRNAs that accumulate and decay during embryogenesis, and are either undetectable or present at low levels in...
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Article
Tobacco single-copy DNA is highly homologous to sequences present in the genomes of its diploid progenitors
We compared the single-copy DNA sequences of the tetraploid tobacco plant, Nicotiana tabacum, with those of its diploid progenitors N. sylvestris and N. tomentosiformis. We observed that 65% of N. sylvestris and
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Article
INTRAVENOUS LIPID INFUSION INCREASES LUNG FLUID FILTRATION IN LAMBS
In adult sheep intravenous infusion of a 10% lipid emulsion at an hourly rate of 0.25 g/kg body weight increases pulmonary microvascular pressure and filtration of fluid into the lungs, causing pulmonary lymph...
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Article
Scientist at the helm
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Chapter
Organization of Soybean Seed Protein Genes and Their Flanking Regions
In this paper we review briefly the research which has been carried out in our lab on the organization of soybean seed protein genes. The genes which we have concentrated our efforts on encode the storage prot...
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Article
Analysis of rat adrenal cells in primary culture separated by velocity sedimentation
Cell separation was used to follow the fate of the cortical cells of the adrenal gland in primary culture, and to assess some of the changes that occur as cells adapt to culture conditions. Primary cultures of...
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Article
Hybridisation of scDNA does not lead to overestimates of mRNA complexity
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Chapter
Structural Gene Expression in Tobacco
Cellular and developmental processes in higher plants differ significantly from those of other eukaryotes. Although plant biology plays a central role in food production and agriculture, our knowledge of the m...
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Article
DNA sequence organization in soybean investigated by electron microscopy
An electron microscopic analysis of the DNA sequence organization in the soybean genome is reported. This analysis employed the gene 32 proteinethidium bromide spreading technique, a procedure which produces s...
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Article
DNA sequence organization in the soybean plant
The arrangement of repetitive and nonrepetitive DNA sequences in the soybean genome was ascertained by a comparison of the reassociation kinetics of short (250 nucleotides) and long (2700 nucleotides) DNA frag...
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Article
DNA sequence organization in the genome of Nicotiana tabacum
The genome of Nicotiana tabacum was investigated by DNA/DNA reassociation for its spectrum of DNA repetition components and pattern of DNA sequence organization. The reassociation of 300 nucleotide DNA fragments ...
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Article
DNA sequence organization in the genomes of five marine invertebrates
The arrangement of repetitive and non-repetitive sequence was studied in the genomic DNA of the oyster (Crassostrea virginica), the surf clam (Spisula solidissima), the horseshoe crab (Limulus polyphemus), a neme...
