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Article
Open AccessWhole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podovirusesto other N4-like phages reveal extensive genetic diversity
Vibrio cholerae O139 Bengal is the only serogroup other than O1 implicated in cholera epidemics. We describe the isolation and characterization of an O139 serogroup-specific phage, vB_VchP_VchO139-I (ϕVchO139-I) ...
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Article
Open AccessWhole genome sequencing of phage resistant Bacillus anthracismutants reveals an essential role for cell surface anchoring protein CsaB in phage AP50c adsorption
Spontaneous Bacillus anthracis mutants resistant to infection by phage AP50c (AP50R) exhibit a mucoid colony phenotype and secrete an extracellular matrix.
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Protocol
Isolation of Knotted DNA from Coliphage P4
Type II topoisomerases readily remove knots from DNA (1). Removal of knots can be assayed by gel electrophoresis (1), and this provides a convenient assay for type II topoisomerase activity, even in crude extract...
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Book
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Article
Viral Transactivation
Transactivation is a term that was coined to describe the induction of lambda phage late gene expression by lambda gene Q, which blocks transcription termination. Transactivation is also used to describe two phen...
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Article
Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids
A set of plasmids that contain fragments of the bacteriophage P4 genome has been constructed by deleting portions of a P4-ColE1 hybrid. A P4 genetic map has been established and related to the physical map by ...
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Article
Control of prokaryotic gene expression
The Operon.
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Chapter
The P 2–P 4 Transactivation System
The interactions between bacteriophage P 2 and its satellite phage P 4 comprise a promising model system for studying the control of transcription because the two phage have relatively small and therefore trac...
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Chapter
Head Size Determination and the Morphogenesis of Satellite Phage P4
Satellite phage P4 is a virus which can only make plaques on E. coli strains which carry P2 or a related helper prophage (Six, 1963; Six and Klug, 1973). P4 requires all the head and tail genes of its helper prop...