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Open AccessDevelopment and evaluation of a candidate reference measurement procedure for detecting 17α-hydroxyprogesterone in dried blood spots using isotope dilution liquid chromatography tandem mass spectrometry
17α-Hydroxyprogesterone (17α-OHP) quantification in dried blood spots (DBS) is essential for newborn screening for congenital adrenal hyperplasia (CAH), which is challenging due to its low physiological concen...
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Article
Open AccessTargeted LC-MS/MS profiling of bile acids reveals primary/secondary bile acid ratio as a novel biomarker for necrotizing enterocolitis
Bile acids (BAs) are involved in the development of necrotizing enterocolitis (NEC), which mainly occurs in preterm infants. We aim to identify the change of BAs in preterm infants and validate its potential v...
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Article
Open AccessUltrasensitive and colorimetric small extracellular vesicles (sEVs) analysis via dual-cycle signal tool
For the clinical diagnosis of diseases and for basic biological research, it is crucial to develop a trustworthy and efficient method for detecting small extracellular vesicles (sEVs) in multiple experimental ...
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Article
Open AccessCorrection to: Influence of data acquisition modes and data analysis approaches on non‑targeted analysis of phthalate metabolites in human urine
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Article
Lab at home: a promising prospect for on-site chemical and biological analysis
The continuing pursuit for a healthy life has led to the urgent need for on-site analysis. In response to the urgent needs of on-site analysis, we propose a novel concept, called lab at home (LAH), for buildin...
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Article
Open AccessInfluence of data acquisition modes and data analysis approaches on non-targeted analysis of phthalate metabolites in human urine
Humans are often exposed to phthalates and their alternatives, on account of their widespread use in PVC as plasticizers, which are associated with harmful human effects. While targeted biomonitoring provides ...
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Article
CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus
This study provides proof of concept of a colorimetric biosensor for influenza H1N1 virus assay based on the CRISPR/Cas13a system and hybridization chain reaction (HCR). Target RNA of influenza H1N1 virus acti...
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Article
Open AccessSimultaneous profiling and quantification of 25 eicosanoids in human serum by ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry
The eicosanoid metabolic pathway is responsible for mediating the production of various inflammatory factors that are closely related to the development and resolution of inflammation. In biological matrices, ...
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Article
Open AccessDetection storage time of mild bruise’s yellow peaches using the combined hyperspectral imaging and machine learning method
To deduce the process of bruise and reduce the number of bruised fruits from the source, the storage time of yellow peaches after bruise should be identified. In order to distinguish the different storage time...
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Article
Antigenic characterization of influenza and SARS-CoV-2 viruses
Antigenic characterization of emerging and re-emerging viruses is necessary for the prevention of and response to outbreaks, evaluation of infection mechanisms, understanding of virus evolution, and selection ...
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Article
Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12a
In this study, Lba Cas12a (Cpf1) as one of the CRISPR systems from Lachnospiraceae bacterium was coupled with a hybridization chain reaction (HCR) to develop an electrochemical biosensor for detecting the pathoge...
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Article
Open AccessDetermination of tyrosine by sodium fluorescein-enhanced ABEI–H2O2–horseradish peroxidase chemiluminescence
In this study, N-(4-aminobutyl)-N-ethylisoluminol (ABEI) was used as an energy donor, while sodium fluorescein was used as an enhancer and energy acceptor, which resulted in it producing resonance energy trans...
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Article
SARS-CoV-2 pandemic: a review of molecular diagnostic tools including sample collection and commercial response with associated advantages and limitations
The unprecedented global pandemic known as SARS-CoV-2 has exercised to its limits nearly all aspects of modern viral diagnostics. In doing so, it has illuminated both the advantages and limitations of current ...
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Article
Correction to: Consecutive and automatic detection of multi-gene mutations from colorectal cancer samples by coupling droplet array-based capillary electrophoresis and PCR-RFLP
The authors would like to call the reader’s attention to the fact that unfortunately the name of Jianzhang Pan was missing as co-author of this contribution.
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Article
Future perspective: high-throughput construction of new ultrasensitive cytokine and virion liquid chips for high-throughput screening (HTS) of anti-inflammatory drugs or clinical diagnosis and treatment of inflammatory diseases
Pathogen–host cell interactions play an important role in many human infectious and inflammatory diseases. Several pathogens, including Escherichia coli (E. coli), Mycobacterium tuberculosis (M. tb), and even the...
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Article
Open AccessRapid determination of S-(+)-linalool in leaf of Cinnamomum osmophloeum ct. linalool using ultrasound-assisted microextraction
Cinnamomum osmophloeum ct. linalool is one chemotype of indigenous cinnamon in Taiwan. Its leaf essential oil (LEO) and main component S-(+)-linalool both possess great anxiolytic activities. The aim of this stud...
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Article
A new immunoassay of serum antibodies against Peste des petits ruminants virus using quantum dots and a lateral-flow test strip
A fast and ultrasensitive test-strip system combining quantum dots (QDs) with a lateral-flow immunoassay strip (LFIAS) was established for detection of Peste des petits ruminants virus (PPRV) antibody. The highly...
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Article
Quality fluctuation detection of an herbal injection based on biological fingerprint combined with chemical fingerprint
Herbal injection is one of the most important preparations of traditional Chinese medicine. More than 130 types of herbal injections are used clinically for 400 million patients annually with total sales of ov...
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Article
Open AccessIdentification of serum biomarkers of hepatocarcinoma through liquid chromatography/mass spectrometry-based metabonomic method
Late diagnosis of hepatocarcinoma (HCC) is one of the most primary factors for the poor survival of patients. Thereby, identification of sensitive and specific biomarkers for HCC early diagnosis is of great im...
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Article
Open AccessNew insights into the formation of volatile compounds in mainstream cigarette smoke
A sampling system has been set up to monitor a group of volatile smoke analytes (nitric oxide, acetaldehyde, acetone, benzene, toluene, 1,3 butadiene, isoprene and carbon dioxide) from mainstream cigarette smo...