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Article
Open AccessReplication dynamics identifies the folding principles of the inactive X chromosome
Chromosome-wide late replication is an enigmatic hallmark of the inactive X chromosome (**). How it is established and what it represents remains obscure. By single-cell DNA replication sequencing, here we sho...
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Article
Single-cell DNA replication profiling identifies spatiotemporal developmental dynamics of chromosome organization
In mammalian cells, chromosomes are partitioned into megabase-sized topologically associating domains (TADs). TADs can be in either A (active) or B (inactive) subnuclear compartments, which exhibit early and l...
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Article
Open AccessThe Eleanor ncRNAs activate the topological domain of the ESR1 locus to balance against apoptosis
MCF7 cells acquire estrogen-independent proliferation after long-term estrogen deprivation (LTED), which recapitulates endocrine therapy resistance. LTED cells can become primed for apoptosis, but the underlyi...
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Protocol
Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles
Recent advances in next-generation sequencing (NGS) and chromosome conformation capture (3C) analysis have led to the development of Hi-C, a genome-wide version of the 3C method. Hi-C has identified new levels...
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Article
Open AccessChromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins
Chromosome higher order structure has been an enigma for over a century. The most important structural finding has been the presence of a chromosome scaffold composed of non-histone proteins; so-called scaffol...
