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Protocol
A T-Linker Strategy for Modification and Directional Cloning of PCR Products
The propensity of Taq polymerase to add 3′-A overhangs (1,2) to polymerase chain reaction (PCR)-amplified DNA has made possible a simple method for cloning PCR products into a T-vector (Invitrogen, San Diego, CA)...
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Chapter
Anti-Acetylcholine Receptor CD4+ T Cells in Myasthenia Gravis: Epitope repertoire and T cell receptor gene usage
Although the symptoms of myasthenia gravis (MG) are due to the binding of auto-antibodies to the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction (reviewed in [1]), the anti-AChR CD4+T cells ...
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Protocol
A T-Linker Strategy for Modification and Directional Cloning of PCR Products
The propensity of Taq polymerase to add 3′-A overhangs (1,2) to PCR-amplified DNA has made possible a simple method for cloning PCR products into a T-vector (Invitrogen [San Diego, CA]; 3–5). Here we present a re...
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Protocol
Designing PCR Primers to Amplify Specific Members or Subgroups of Multigene Families
Multigene families throw a particular twist into the problem of polymerase chain reaction (PCR) primer design. One frequently needs primers that will not only amplify the sequence of interest, but at the same ...
