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Article
Enhancement of transforming growth factor-α synthesis in multicellular tumour spheroids of A431 squamous carcinoma cells
Multicellular tumour spheroids are cellular aggregates that can be prepared from many types of tumour cells. These three-dimensional structures provide a model for analysing the effects of cell-cell contact an...
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Article
Hypoxia-induced drug resistance: comparison to P-glycoprotein-associated drug resistance
In this report, we investigate several examples of hypoxia-induced drug resistance and compare them with P-glycoprotein associated multidrug resistance (MDR). EMT6/Ro cells exposed to drugs in air immediately ...
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Article
Differences in EGF related radiosensitisation of human squamous carcinoma cells with high and low numbers of EGF receptors
Previous studies have shown that the presence of epidermal growth factor (EGF) after irradiation enhanced the radiosensitivity of CaSki cells. To examine the role of EGF receptor density and related growth res...
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Article
The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells
Chronic hypoxia increases the expression of a set of stress proteins (oxygen regulated proteins or ORPs) which is implicated in the development of drug resistance and radiation sensitivity in tumour cells. Fiv...
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Article
Enhanced synthesis of stress proteins caused by hypoxia and relation to altered cell growth and metabolism
Cultured cells maintained in very low oxygen levels alter their structure, metabolism and genetic expression. Culture conditions for cells were modified to minimise variation of nutrients and to allow normal s...
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Article
Changes in growth characteristics and macromolecular synthesis on recovery from severe hypoxia
Chinese hamster ovary cells subjected to severe hypoxia stop growing. When oxygen was reintroduced growth resumed, but at a slower rate. The longer the hypoxic stress, the slower the recovery growth rate. Six ...
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Article
Changes in cellular glutathione content during adriamycin treatment in human ovarian cancer – a possible indicator of chemosensitivity
Patients with ovarian cancer often respond well to combination chemotherapy initially but the majority eventually relapse when, with further treatment, the initially successful regimen proves ineffectual. The ...
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Article
Growth and radiation sensitivity of the MLS human ovarian carcinoma cell line grown as multicellular spheroids and xenografted tumours
The growth characteristics and the radiation sensitivity of multicellular spheroids of the MLS human ovarian carcinoma cell line grown in spinner culture in atmospheres of 5% CO2 in air or 5% CO2, 5% O2 and 90...
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Article
Intracapillary HbO2 saturations in murine tumours and human tumour xenografts measured by cryospectrophotometry: relationship to tumour volume, tumour pH and fraction of radiobiologically hypoxic cells
Frequency distributions for intracapillary HbO2 saturation were determined for two murine tumour lines (KHT, RIF-1) and two human ovarian carcinoma xenograft lines (MLS, OWI) using a cryospectrophotometric met...
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Article
Dependence of misonidazole binding on factors associated with hypoxic metabolism
The binding of misonidazole (MISO) to macromolecules in hypoxic cells is believed to require metabolic reduction. Several factors in the cells' environment, such as pH, glucose, lactate and MISO concentration ...
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Article
Characterization of radiation sensitivity of human squamous carcinoma A431 cells
Studies have been performed to investigate the radiosensitivity of human squamous carcinoma cells. A431 cells were grown in vitro as exponential and fed-plateau monolayer cultures or as multicellular spheroids...
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Article
Comparative pO2 measurements in cell spheroids cultured with different techniques
