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Chapter
Introduction, The Importance of Nucleic Acids in Diagnostics
Molecular diagnostics comprises a rapidly develo** field in clinical and veterinary medicine, plant pathology, and food safety. Techniques have become more rapid, cheaper, and more accurate during the last d...
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Chapter
Primers and Probes
Nucleic acid probes are the crucial reagents to detect specific nucleic acid sequences by means of hybridisation. Visualisation of a specific hybridised probe, either in solution or attached to a solid phase, ...
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Chapter
Principles of PCR
PCR is used to generate large amounts of nucleic acid from small amounts or target, using either conventional or real-time technology. Essential reagents are a thermostable DNA-dependant DNA polymerase, synthe...
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Chapter
Basic Principles of Nucleic Acid Hybridisation
Hybridisation is the key process in many molecular diagnostic techniques. Hybridisation is based on Watson-Crick base pairing between specific sequences in a native nucleic acid fragment (target) and a (mostly...
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Article
Antimicrobial susceptibility testing of Gram-positive and -negative bacterial isolates directly from spiked blood culture media with Raman spectroscopy
Patients suffering from bacterial bloodstream infections have an increased risk of develo** systematic inflammatory response syndrome (SIRS), which can result in rapid deterioration of the patients’ health. ...
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Article
Open AccessIn vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization
A broth for the screening of group B streptococcal (GBS) carriage during pregnancy is about to be introduced. Simulating conditions in everyday practice, we have compared the sensitivity of this Granada tube b...
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Article
Open AccessExternal quality assessment of the molecular diagnostics and genoty** of meticillin-resistant Staphylococcus aureus
Two multicentre external quality assessments (EQA) for the molecular detection and genoty** of meticillin-resistant Staphylococcus aureus (MRSA) were arranged. Firstly, 11 samples containing various amounts of ...
