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Article
Engineering isoflavone metabolism with an artificial bifunctional enzyme
Plant secondary metabolism has been a focus of research in recent years due to its significant roles in plant defense and in human medicine and nutrition. A protein engineering strategy was designed to more ef...
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Chapter
The Stereochemistry of Flavonoids
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Chapter
Metabolomics and Gene Identification in Plant Natural Product Pathways
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Chapter
Identification and Characterization of a Binding Protein for N-Acylethanolamines in Membranes of Tobacco Cells
N-acylethanolamines (NAEs) are endogenous constituents of various plant tissues and are derived from the membrane phospholipid, N-acylphosphatidylethanolamine (NAPE), by the action of phospholipase...
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Article
Structure and mechanism of chalcone synthase-like polyketide synthases
Polyketide synthases (PKS) produce an array of natural products with different biological activities and pharmacological properties by varying the starter and extender molecules that form the final polyketide....
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Article
Basal activity of the hypothalamic-pituitary-adrenal axis and cognitive function in anorexia nervosa
Anorexia nervosa is associated with abnormalities in neuroendocrine function including sustained hypercortisolism, which has been shown elsewhere to be associated with impairment of function in learning, memo...
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Chapter
Nitric oxide signalling in the plant hypersensitive disease resistance response
Plants have evolved several mechanisms to prevent invasion of their tissues by pathogens. A common feature of disease resistance is the hypersensitive response at and immediately surrounding infection sites, w...
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Chapter
Expression of Genes for Enzymes of the Flavonoid Biosynthetic Pathway in the Early Stages of the Rhizobium-Legume Symbiosis
Isoflavonoids/flavonoids have been found to be involved in a number of critical plant activities, such as plant defense, pollen development, pigmentation, and the Rhizobium-legume symbiosis. The biosynthetic path...
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Chapter
Cephalocereus senilis (Old-Man-Cactus): In Vitro Culture and the Elicitation of Flavonoids
The abundance of cacti in arid lands is, at least in part, due to several biochemical and anatomical adaptations that cacti have for minimizing water loss. To what degree members of the Cactaceae have evolved ...
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Article
Plant expression cassettes for enhanced translational efficiency
A number of plant expression cassettes have been constructed that are designed to ensure efficient ribosome recruitment, thereby improving translational efficiency. For this purpose the casettes employ the unt...
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Chapter
Emerging Strategies for Enhancing Crop Resistance to Microbial Pathogens
There are marked differences in the pattern of host gene expression in incompatible plant: microbial pathogen interactions compared with compatible interactions, associated with the elaboration of inducible de...
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Chapter
Properties of Plant Defense Gene Promoters
Elaboration of active responses to mechanical damage, elicitors or microbial attack involves transcriptional activation of batteries of defense genes. Data from recent functional analysis of the promoters of t...
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Article
Sequence analysis of a chalcone isomerase cDNA of Phaseolus vulgaris L.
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Chapter
Studies of the Potential for Expression of Nitrogenase Fe-Protein in Cells of Higher Plants
The potential for expression of the nitrogenase Fe-protein in Nicotiana tabacum has been examined by construction of hybrid nifH and nifM genes which should target their polypeptide products to the plant chloropl...
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Article
Role of metal ions in negative regulation of nitrogen fixation by the nifL gene product from Klebsiella pneumoniae
The ability of the Klebsiella pneumoniae nifL gene product to antagonise NIFA mediated transcriptional activation from the nifH promoter in vivo was inhibited either by metal deprivation, or by the presence of th...
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Chapter and Conference Paper
Regulation of Gene Expression in Biologically Stressed Bean Cell Cultures
For many years, an important aim of plant biotechnology has been to manipulate cultured plant cells in order to increase the metabolic flux into specific pathways of secondary product formation. In most cases ...
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Article
Effect of inoculation withKlebsiella oxytoca andEnterobacter cloacae on dinitrogen fixation by rice-bacteria associations
The influence ofKlebsiella oxytoca andEnterobacter cloacae inoculation on dinitrogen fixation by the rice-bacteria association was examined in pots in a greenhouse. For inoculation,K. oxytoca NG13 isolated from a...
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Article
L-Phenylalanine ammonia-lyase fromPhaseolus vulgaris: Modulation of the levels of active enzyme bytrans-cinnamic acid
The extractable activity ofl-phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) in cell suspension cultures of bean (Phaseolus vulgaris) is greatly induced following exposure to an elicitor preparation from the cell w...
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Article
Regulation of the nitrogen fixation genes inKlebsiella pneumoniae: Implications for genetic manipulation
The nitrogen-fixing bacteriumKlebsiella pneumoniae has provided a model system for analysis of the organisation and regulation of nitrogen fixation (nif) genes in diazotrophs. Expression of the 17nif genes inKleb...
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Chapter
Regulation of the nitrogen fixation genes in Klebsiella pneumoniae: Implications for genétic manipulation
The nitrogen-fixing bacterium Klebsiella pneumoniae has provided a model system for analysis of the organisation and regulation of nitrogen fixation (nif) genes in diazotrophs. Expression of the 17 nif genes in K...
