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Chapter
Mutagenesis
Bacterial plasmids are autonomously replicating mini chromosomes (replicons) which contain genetic information for many different properties, including the production of toxins and adhesion antigens, resistanc...
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Chapter
Basic Methods
Gene transfer in bacteria occurs by three main processes: transformation and transfection, which involve the direct uptake of free DNA by bacteria; transduction, which involves bacteriophage mediated transmiss...
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Chapter
Gene Cloning
Genetic recombination is the fundamental biological process of exchange of genetic information between different chromosomes. Its constant activity during evolution has enabled the large number of spontaneousl...
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Chapter
Bioluminescent REC A Mutants of Rhizobium as Model Organisms in Risk Assessment Studies
A major concern about the deliberate release of genetically modified microorganisms (GEMMOs) is that the released organism exerts a potential disruptive effect on the biotic community of its target eco-system....
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Article
Prenatal hypertrophic cardiomyopathy and pericardial effusion in carbohydrate-deficient glycoprotein syndrome
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Article
Iodine species and the endocrine system: thyroid hormone levels in adult Danio rerio and develo** Xenopus laevis
Recently a new approach for the analysis of iodinated organic species in human serum has been developed using liquid chromatography–inductively coupled plasma–mass spectrometry (LC–ICP–MS). This method enable...
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Article
Congenital disorder of glycosylation (CDG) type Ie. A new patient
Summary: CDG Ie is caused by a deficiency of dolichol-phosphate-mannose synthase 1 (DPM1), an enzyme involved in N-glycan assembly in the endoplasmic reticulum. Three proteins are known t...
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Article
Investigation of element distribution and homogeneity of TXRF samples using SR-micro-XRF to validate the use of an internal standard and improve external standard quantification
Total reflection X-ray fluorescence analysis (TXRF) offers a nondestructive qualitative and quantitative analysis of trace elements. Due to its outstanding properties TXRF is widely used in the semiconductor i...
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Article
Open AccessProgress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study
In the present paper, X-ray fluorescence microscopy was applied to follow the processes occurring in rat hippocampal formation during the post-seizure period. In the study, one of the status epilepticus animal mo...
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Article
Open AccessElemental changes in the hippocampal formation following two different formulas of ketogenic diet: an X-ray fluorescence microscopy study
The main purpose of the following study was the determination of elemental changes occurring within hippocampal formation as a result of high-fat and carbohydrate-restricted ketogenic diet (KD). To realize it...