Skip to main content

4 Result(s)

within Qingyi Lin Remove this filter Developmental Biology Remove this filter

and

  1. No Access

    Article

    Effects of centrifugation treatment before electroporation on gene editing in pig embryos

    Genetic mosaicism, characterized by multiple genotypes within an individual, is considered an obstacle to CRISPR/Cas9 genome editing in animal models. Despite the various strategies for minimizing mosaic mutat...

    Bin Liu, Manita Wittayarat, Koki Takebayashi… in In Vitro Cellular & Developmental Biology … (2024)

  2. No Access

    Article

    Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes

    The delivery of CRISPR/Cas ribonucleoprotein (RNP) complexes is gaining attention owing to its high cleavage efficiency and reduced off-target effects. Although RNPs can be delivered into porcine zygotes via e...

    Qingyi Lin, Koki Takebayashi, Nanaka Torigoe… in In Vitro Cellular & Developmental Biology … (2024)

  3. No Access

    Article

    Programmed cell death-1-modified pig developed using electroporation-mediated gene editing for in vitro fertilized zygotes

    Programmed cell death-1 (PD-1) is an immunoinhibitory receptor required to suppress inappropriate immune responses such as autoimmunity. Immune checkpoint antibodies that augment the PD-1 pathway lead to immun...

    Thanh-Van Nguyen, Lanh Thi Kim Do… in In Vitro Cellular & Developmental Biology … (2024)

  4. No Access

    Article

    One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs

    In the present study, we investigated whether electroporation could be used for one-step multiplex CRISPR/Cas9-based genome editing, targeting IL2RG and GHR in porcine embryos. First, we evaluated and selected gu...

    Maki Hirata, Manita Wittayarat… in In Vitro Cellular & Developmental Biology … (2020)