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Article
Effects of centrifugation treatment before electroporation on gene editing in pig embryos
Genetic mosaicism, characterized by multiple genotypes within an individual, is considered an obstacle to CRISPR/Cas9 genome editing in animal models. Despite the various strategies for minimizing mosaic mutat...
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Article
Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes
The delivery of CRISPR/Cas ribonucleoprotein (RNP) complexes is gaining attention owing to its high cleavage efficiency and reduced off-target effects. Although RNPs can be delivered into porcine zygotes via e...
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Article
Programmed cell death-1-modified pig developed using electroporation-mediated gene editing for in vitro fertilized zygotes
Programmed cell death-1 (PD-1) is an immunoinhibitory receptor required to suppress inappropriate immune responses such as autoimmunity. Immune checkpoint antibodies that augment the PD-1 pathway lead to immun...
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Article
Tanshinone IIA (TSIIA) represses the progression of non-small cell lung cancer by the circ_0020123/miR-1299/HMGB3 pathway
Tanshinone IIA (TSIIA), a multi-pharmaceutical compound, has been demonstrated to have anti-tumor properties. This study explores the potential regulatory mechanism of TSIIA on non-small cell lung cancer (NSCL...
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Article
Open AccessMicroencapsulated phase change material through cellulose nanofibrils stabilized Pickering emulsion templating
Phase change materials (PCMs) possess remarkable capability to store and release substantial amounts of energy during the processes of melting and crystallization across a wide temperature range, thus holding ...
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Article
GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system
Pigs are excellent large animal models with several similarities to humans. They provide valuable insights into biomedical research that are otherwise difficult to obtain from rodent models. However, even if m...
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Article
Open AccessGeneration of mutant pigs by lipofection-mediated genome editing in embryos
The specificity and efficiency of CRISPR/Cas9 gene-editing systems are determined by several factors, including the mode of delivery, when applied to mammalian embryos. Given the limited time window for delive...
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Article
Open AccessTiming and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
Lipofection-mediated introduction of the CRISPR/Cas9 system in porcine zygotes provides a simple method for gene editing, without requiring micromanipulation. However, the gene editing efficiency is inadequate...
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Article
Open AccessComparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study...
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Article
One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs
In the present study, we investigated whether electroporation could be used for one-step multiplex CRISPR/Cas9-based genome editing, targeting IL2RG and GHR in porcine embryos. First, we evaluated and selected gu...
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Article
Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation
The CRISPR/Cas9 system now allows for unprecedented possibilities of genome editing. However, there are some limitations, including achieving efficient one-step multiple genome targeting to save costs, time, a...
