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Open AccessTiming and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
Lipofection-mediated introduction of the CRISPR/Cas9 system in porcine zygotes provides a simple method for gene editing, without requiring micromanipulation. However, the gene editing efficiency is inadequate...
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Open AccessComparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study...