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  1. Article

    Open Access

    Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events

    Lipofection-mediated introduction of the CRISPR/Cas9 system in porcine zygotes provides a simple method for gene editing, without requiring micromanipulation. However, the gene editing efficiency is inadequate...

    Qingyi Lin, Quynh Anh Le, Koki Takebayashi in BMC Research Notes (2021)

  2. Article

    Open Access

    Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

    Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study...

    Quynh Anh Le, Fuminori Tanihara, Manita Wittayarat, Zhao Namula in BMC Research Notes (2021)