20 Result(s)
within
Qingsheng Qi
Applied Microbiology
-
Article
Construction of a novel system for cell surface display of heterologous proteins on Pichia pastoris
A versatile vector was developed for heterologous proteins display on the cell surface of Pichia pastoris using the C-terminal half of α-agglutinin from Saccharomyces cerevisiae as a membrane anchor under the con...
-
Article
A two-step fermentation process for efficient production of penta-N-acetyl-chitopentaose in recombinant Escherichia coli
The nodC gene from Mesorhizobium loti was cloned into E. coli, leading to production of chitin oligosaccharides (COs)—mainly penta-N-acetyl-chitopentaose. A two-step fermentation procedure was then developed whic...
-
Article
Open AccessComplete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological application
Poly-β-hydroxybutyrate (PHB) mobilization in bacteria has been proposed as a mechanism that can benefit their host for survival under stress conditions. Here we reported for the first time that a stress-induced s...
-
Article
A marine bacterium accumulates polyhydroxyalkanoate consisting of mainly 3-hydroxydodecanoate and 3-hydroxydecanoate
A deep-sea psychrotrophic bacterium Pseudoaltermonas sp. SM9913, isolated from abyssalbenthic sediments in Bohai Sea, was found to be able to synthesize polyhydroxyalkanoate (PHA) polymer consisting of mainly 3-h...
-
Article
Open AccessA strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli
For metabolic engineering, many rate-limiting steps may exist in the pathways of accumulating the target metabolites. Increasing copy number of the desired genes in these pathways is a general method to solve ...
-
Article
Small RNA RyhB as a potential tool used for metabolic engineering in Escherichia coli
Small RNA (RyhB) was overexpressed artificially using an arabinose-inducible system in Escherichia coli and resulted in more succinate (7-fold) accumulation, which suggested that RyhB had a strong effect on sdhCD...
-
Article
Open AccessOne-step of tryptophan attenuator inactivation and promoter swap** to improve the production of L-tryptophan in Escherichia coli
L-tryptophan is an aromatic amino acid widely used in the food, chemical and pharmaceutical industries. In Escherichia coli, L-tryptophan is synthesized from phosphoenolpyruvate and erythrose 4-phosphate by enzym...
-
Article
Extending homologous sequence based on the single gene mutants by one-step PCR for efficient multiple gene knockouts
Multiple gene knockouts play an important role in metabolic engineering. The flanked homology length, homologous to the region adjacent to the target gene, of the knockout fragments has a great effect on the e...
-
Article
Open AccessIdentification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli
The Gram-negative bacterium Escherichia coli has been widely used as a cell factory for the production of proteins and specialty chemicals because it is the best characterized host with many available expression ...
-
Article
Open AccessProphage recombinases-mediated genome engineering in Lactobacillus plantarum
Lactobacillus plantarum is a food-grade microorganism with industrial and medical relevance belonging to the group of lactic acid bacteria (LAB). Traditional strategies for obtaining g...
-
Article
Open AccessConstruction of cellulose-utilizing Escherichia coli based on a secretable cellulase
The microbial conversion of plant biomass into value added products is an attractive option to address the impacts of petroleum dependency. The Gram-negative bacterium Escherichia coli is commonly used as host fo...
-
Article
Open AccessEngineering Corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose
Corynebacterium glutamicum is generally regarded as a safe microorganism and is used to produce many biochemicals, including l-glutamate. 5-Aminolevulinic acid (ALA) is an l-glutamate ...
-
Article
Open AccessEasy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system
Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously sha...
-
Article
Open AccessNovel technologies combined with traditional metabolic engineering strategies facilitate the construction of shikimate-producing Escherichia coli
Shikimate is an important intermediate in the aromatic amino acid pathway, which can be used as a promising building block for the synthesis of biological compounds, such as neuraminidase inhibitor Oseltamivir...
-
Article
Open AccessThe phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due ...
-
Article
Open AccessEngineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers
Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their su...
-
Article
Open AccessIdentification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4
In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to...
-
Article
Open AccessEngineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae
With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors construct...
-
Article
Open AccessFine tuning the glycolytic flux ratio of EP-bifido pathway for mevalonate production by enhancing glucose-6-phosphate dehydrogenase (Zwf) and CRISPRi suppressing 6-phosphofructose kinase (PfkA) in Escherichia coli
Natural glycolysis encounters the decarboxylation of glucose partial oxidation product pyruvate into acetyl-CoA, where one-third of the carbon is lost at CO2. We previously constructed a carbon saving pathway, EP...
-
Article
Open AccessCharacterization of Salmonella endolysin XFII produced by recombinant Escherichia coli and its application combined with chitosan in lysing Gram-negative bacteria
Salmonella is a common foodborne pathogen, which can cause intestinal diseases. In the last decades, the overuse of antibiotics has led to a pandemic of drug-resistant bacterial infections. To tackle the burden ...
