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Article
Open AccessPhenolic compounds induce ferroptosis-like death by promoting hydroxyl radical generation in the Fenton reaction
Phenolic compounds are industrially versatile chemicals, also the most ubiquitous pollutants. Recently, biosynthesis and biodegradation of phenols has attracted increasing attention, while phenols’ toxicity is...
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Article
Open AccessGenome-scale transcriptional activation by non-homologous end joining-mediated integration in Yarrowia lipolytica
Genome-scale screening can be applied to efficiently mine for unknown genes with phenotypes of interest or special functions. It is also useful to identify new targets for engineering desirable properties of c...
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Article
Development of genetic markers in Yarrowia lipolytica
The oleaginous yeast Yarrowia lipolytica represents a potential microbial cell factory for the recombinant production of various valuable products. Currently, the commonly used selection markers for transformatio...
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Protocol
In Vivo DNA Assembly Using the PEDA Method
Simple and efficient DNA assembly methods have been widely used in synthetic biology. Here, we provide the protocol for the recently developed PEDA (phage enzyme-assisted in vivo DNA assembly) method for direc...
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Article
Open AccessReconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica
Succinic acid (SA) is an important C4-dicarboxylic acid. Microbial production of SA at low pH results in low purification costs and hence good overall process economics. However, redox imbalances limited SA bi...
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Article
Open AccessConstruction of cascade circuits for dynamic temporal regulation and its application to PHB production
To maximize the production capacity and yield of microbial cell factories, metabolic pathways are generally modified with dynamic regulatory strategies, which can effectively solve the problems of low biologic...
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Article
Open AccessUsing a synthetic machinery to improve carbon yield with acetylphosphate as the core
In microbial cell factory, CO2 release during acetyl-CoA production from pyruvate significantly decreases the carbon atom economy. Here, we construct and optimize a synthetic carbon conserving pathway named as Se...
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Article
Open AccessA synthetic population-level oscillator in non-microfluidic environments
Synthetic oscillators have become a research hotspot because of their complexity and importance. The construction and stable operation of oscillators in large-scale environments are important and challenging. ...
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Article
Biodegradation of polyurethane by the microbial consortia enriched from landfill
Polyurethanes (PU) are one of the most used categories of plastics and have become a significant source of environmental pollutants. Degrading the refractory PU wastes using environmentally friendly strategies...
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Article
Open AccessHeme biosensor-guided in vivo pathway optimization and directed evolution for efficient biosynthesis of heme
Heme has attracted much attention because of its wide applications in medicine and food. The products of genes hemBCDEFY convert 5-aminolevulinic acid to protoporphyrin IX (PPIX; the immediate precursor of heme);...
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Article
Open AccessEngineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock
β-Farnesene is a sesquiterpene with versatile industrial applications. The production of β-farnesene from waste lipid feedstock is an attractive method for sustainable production and recycling waste oil. Yarrowia...
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Article
Open AccessConstruction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli
With the development of metabolic engineering and synthetic biology, the biosynthesis of aromatic compounds has attracted much attention. Cinnamylamine is an aromatic compound derived from l-phenylalanine, which ...
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Article
Open AccessCharacterization of Salmonella endolysin XFII produced by recombinant Escherichia coli and its application combined with chitosan in lysing Gram-negative bacteria
Salmonella is a common foodborne pathogen, which can cause intestinal diseases. In the last decades, the overuse of antibiotics has led to a pandemic of drug-resistant bacterial infections. To tackle the burden ...
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Article
Open Accessα-Farnesene production from lipid by engineered Yarrowia lipolytica
Producing high value-added products from waste lipid feedstock by microbial cell factory has great advantages to minimize the pollution as well as improve the economic value of wasted oils and fats. Yarrowia lipo...
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Article
Open AccessEngineering of Yarrowia lipolytica transporters for high-efficient production of biobased succinic acid from glucose
Succinic acid (SA) is a crucial metabolic intermediate and platform chemical. Development of biobased processes to achieve sustainable SA production has attracted more and more attention in biotechnology indus...
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Article
Identification of phage recombinase function unit in genus Corynebacterium
Phage recombinase function unit (PRFU) plays a key role in the life cycle of phage. Repurposing this system such as lambda-Redαβ or Rac-RecET for recombineering has gained success in Escherichia coli. Previous st...
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Article
Open AccessFine tuning the glycolytic flux ratio of EP-bifido pathway for mevalonate production by enhancing glucose-6-phosphate dehydrogenase (Zwf) and CRISPRi suppressing 6-phosphofructose kinase (PfkA) in Escherichia coli
Natural glycolysis encounters the decarboxylation of glucose partial oxidation product pyruvate into acetyl-CoA, where one-third of the carbon is lost at CO2. We previously constructed a carbon saving pathway, EP...
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Article
Open AccessEngineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae
With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors construct...
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Article
Open AccessAn acid-tolerance response system protecting exponentially growing Escherichia coli
The ability to grow at moderate acidic conditions (pH 4.0–5.0) is important to Escherichia coli colonization of the host’s intestine. Several regulatory systems are known to control acid resistance in E. coli, en...
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Article
Open AccessEngineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene
Yarrowia lipolytica, a non-traditional oil yeast, has been widely used as a platform for lipid production. However, the production of other chemicals such as terpenoids in engineered Y. lipolytica is still low. α...
