333 Result(s)
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Article
Synthesis and characterization of cleavable cationic surfactants with a 1,3-dioxane ring
A series of cationic cleavable surfactants was prepared by condensation of 2-alkyl-1,3-propanediols with 3-bromopropanal, followed by reaction with triethylamine. Each surfactant is a mixture of two diastereom...
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Article
Separation of the two-locus inheritance of photoperiod sensitive genic male sterility in rice and precise map** the pms3 locus
Previous studies determined that photoperiod sensitive genic male sterility(PSGMS) in rice was controlled in most of the crosses by two classical duplicated loci, pms1 and pms3, located on chromosomes 7 and12, re...
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Purification and Characterization of an Extracellular Alkaline Serine Protease with Dehairing Function from Bacillus pumilus
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Efficient esterification of sorbitan oleate by lipase in a solvent-free system
The lipase-catalyzed esterification of sorbitan with oleic acid in a solvent-free system to form sorbitan oleate (commercial name Span80) was studied as a feasible approach aimed at meeting the demand for suga...
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A modified hybridization protocol for low-density diagnostic DNA microarrays
To promote the application of DNa microarrays for clinical diagnosis, the problems of cross-hybridization and low signal intensity in the hybridization processes has been addressed. We tested a new hybridizati...
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Article
Production and purification of a novel antibiotic peptide, adenoregulin, from a recombinant Escherichia coli
Adenoregulin is a member of dermaseptin family which are vertebrate antibiotic peptides having lethal effects against a broad spectrum of bacteria, fungi and protozoa. The 99 bp adenoregulin gene was cloned in...
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Article
Measuring gene flow in the cultivation of transgenic cotton (Gossypium hirsutum L.)
Transgenic Bt cotton NewCott 33B and transgenic tfd A cotton TFD were chosen to evaluate pollen dispersal frequency and distance of transgenic cotton (Gossypium hirsutum L.) in the Huanghe Valley Cotton-producing...
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Changes of Bt Toxin in the Rhizosphere of Transgenic Bt Cotton and its Influence on Soil Functional Bacteria
The concentrations of Bacillus thuringensis (Bt) toxin released from root exudation of Bt cotton were measured by an enzyme-linked immunosorbent assay (ELISA), and its impacts on the numbers of culturable functio...
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Article
Using tandem mass spectrometry to predict chemical transformations of 2-pyrimidinyloxy-N-arylbenzyl amine derivatives in solution
Tandem mass spectrometry is used to predict the chemical transformations of 2-pyrimidinyloxy-N-arylbenzyl amine derivatives. Compound 1, N-2-2-4,6- dimethoxypyrimidin-2-yloxy benzylamino phenyl benzamide was sele...
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Integrating metabolomics into a systems biology framework to exploit metabolic complexity: strategies and applications in microorganisms
As an important functional genomic tool, metabolomics has been illustrated in detail in recent years, especially in plant science. However, the microbial category also has the potential to benefit from integra...
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Article
Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila
Penicillin G acylase (PGA) from Kluyvera citrophila immobilized on Amberzyml was used for enantioselective hydrolysis of N-phenylacetylated-dl-tert-leucine (N-Phac-dl-Tle) to produce l-tert-leucine (l-Tle). The e...
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Article
Identification and fine map** of AvrPi15, a novel avirulence gene of Magnaporthe grisea
Avirulence of Magnaporthe grisea isolate CHL346 on rice cultivar GA25 was studied with 242 ascospore progenies derived from the cross CHL346 × CHL42. Segregation analysis of the avirulence in the progeny populati...
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Article
Expression of truncated phosphoproteins of Nipah virus and Hendra virus in Escherichia coli for the differentiation of henipavirus infections
The genus Henipavirus in the family Paramyxoviridae compromises two newly identified dangerous pathogens, Nipah virus and Hendra virus. Phosphoprotein of the two viruses is one of the major immunodominant antigen...
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Article
The Arabidopsis thaliana AT PRP39-1 gene, encoding a tetratricopeptide repeat protein with similarity to the yeast pre-mRNA processing protein PRP39, affects flowering time
Flowering is regulated by a network integrated from four major pathways, including the photoperiod, vernalization, gibberellin, and autonomous pathways. RNA processing within the autonomous pathway is well kno...
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The Pik m gene, conferring stable resistance to isolates of Magnaporthe oryzae, was finely mapped in a crossover-cold region on rice chromosome 11
The Pik m gene in rice confers a high and stable resistance to many isolates of Magnaporthe oryzae collected from southern China. This gene locus was ro...
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Article
Knockout of the gene (ste15) encoding a glycosyltransferase and its function in biosynthesis of exopolysaccharide in Streptomyces sp. 139
Streptomyces sp. 139 produces a novel exopolysaccharide (EPS) designated Ebosin which has antagonistic activity for IL-1R in vitro and remarkable anti-rheumatic arthritis activity in vivo...
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Article
Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice
GA 20-oxidase (GA20ox) is a regulatory enzyme for the syntheses of biologically active GAs in plants. The loss-of-function mutations in OsGA20ox2 of rice (Oryza sativa L.) generate the well-known Green Revolution...
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Article
Vitrification–cryopreservation, an efficient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips
Huanglongbing disease (HLB), caused by Candidatus Liberobacter asiaticus, constitutes a most serious problem for the Chinese citrus industry. In this work, the use of vitrification-cryopr...
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Article
Two-photon Absorption Properties of 9,10-Disubstituted 2,6-Bis(p-dihexylaminostyryl)Anthracene Derivatives. Effect of 9,10-Substituents
A series of 2,6-bis(p-dihexylaminostyryl)anthracence derivatives having phenyl, styryl, and phenylethynyl groups at 9,10-positions (1−4) have been synthesized and their two-photon cross-sections were determined. ...
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Article
Characterization of a Rolling-Circle Replication Plasmid pLR1 from Lactobacillus plantarum LR1
A cryptic plasmid from Lactobacillus plantarum LR1, designated pLR1, was sequenced and characterized. It consisted of a 2066-bp circular molecule with a G + C content of 52.7%. The plasmid pLR1 was predicted to c...