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Article
A highly sensitive fluorescence biosensor for aflatoxins B1 detection based on polydiacetylene liposomes combined with exonuclease III-assisted recycling amplification
A fluorescence biosensor for determination of aflatoxin B1 (AFB1) based on polydiacetylene (PDA) liposomes and exonuclease III (EXO III)-assisted recycling amplification was developed. The AFB1 aptamer partially ...
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Article
Simultaneous detection of AFB1 and aflD gene by “Y” shaped aptamer fluorescent biosensor based on double quantum dots
The developed method for simultaneous detection of aflatoxin B1 (AFB1) and aflD genes can effectively monitor from the source and reduce the safety problems and economic losses caused by the production of aflatox...
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Article
Multiplex SERS-based lateral flow assay for one-step simultaneous detection of neomycin and lincomycin in milk
Neomycin and lincomycin are widely used in the treatment of infections illnesses. However, the irrational and inappropriate of antibiotics contributes to threaten human health seriously. We developed a rapid a...
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Article
A Novel Fluorescent FRET Hairpin Probe Switch for aflD Gene Detection in Real Fermented Soybean Paste
The development of detection method for aflatoxin gene cluster in Aspergillus flavus and Aspergillus parasiticusis is an effective approach to prevent the widespread of aflatoxins, which are considered as extreme...
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Article
A SERS-based multiple immuno-nanoprobe for ultrasensitive detection of neomycin and quinolone antibiotics via a lateral flow assay
The authors describe an ultrasensitive method for simultaneous detection of neomycin (NEO) and quinolones antibiotics (QNS). It is based on the use of (a) two immuno-nanoprobes (a probe for NEO and a probe for...
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Article
Development of an Indirect Competitive ELISA Kit for the Detection of Soybean Allergenic Protein Gly m Bd 28K
The complementary DNA (cDNA) sequence of Gly m Bd 28K was expressed in Escherichia coli BL21 (DE3) as an inclusion body under the induction of 1.0 mmol/L of isopropyl β-D-thiogalactoside (IPTG). The inclusion bod...
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Article
Development of an Indirect Competitive ELISA Kit for the Rapid Detection of Benzopyrene Residues
This paper presents the generation of monoclonal antibodies (mAbs) with high specificity against benzopyrene (BaP) through cell fusion procedures, and the development of mAb-based indirect competitive enzyme-l...