629 Result(s)
within
Qian** Li
Life Sciences
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Article
The structure of the nucleosome core particle of chromatin in chicken erythrocytes visualized by using atomic force microscopy
The structure of the nuclosome core particle of chromatin in chicken erythrocytes has been examined by using AFM. The 146 bp of DNA wrapped twice around the core histone octamer are clearly visualized. Both th...
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The In Vitro Reconstitution of Nucleosome and its Binding Patterns with HMG1/2 and HMG14/17 Proteins
Using atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers wer...
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Article
Construction and application of fusion proteins of d-amino acid oxidase and glutaryl-7-aminocephalosporanic acid acylase for direct bioconversion of cephalosporin C to 7-aminocephalosporanic acid
Two fusion proteins of d-amino acid oxidase (DAAO) and glutaryl-7-aminocephalosporanic acid acylase (GLA) were designed to simplify the bioconversion process of cephalosporin C to 7-aminocephalosporanic acid (...
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Article
Dysregulation of HSG triggers vascular proliferative disorders
Vascular proliferative disorders, such as atherosclerosis and restenosis, are the most common causes of severe cardiovascular diseases, but a common molecular mechanism remains elusive. Here, we identify and c...
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Article
Abstract of Poster Presentation
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Article
Chemistry and Pharmacology of Gynostemma pentaphyllum
In traditional Chinese medicine, Gynostemma pentaphyllum (Thunb.) Makino is a herbal drug of extreme versatility and has been extensively researched in China. The dammarane saponins isolated from Gynostemma penta...
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Low serum selenium is associated with anemia among older women living in the community
Anemia is common among older adults, and a substantial proportion of anemia in the older population is of indeterminate cause. Low selenium levels have been associated with anemia in animals, but this relation...
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Biotransformation of tolbutamide to 4′-hydroxytolbutamide by the fungus Cunninghamella blakesleeana
The hypoglycemic drug tolbutamide is commonly used as a probe drug to evaluate CYP2C9 enzyme activity in terms of production of 4′-hydroxytolbutamide. In the present study, an initial screening of seven filame...
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A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22
This protocol outlines a methodology for the preparation and characterization of three RNA-specific fluorescent probes (E36, E144 and F22) and their use in live cell imaging. It describes a detailed procedure ...
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Identification of a cytochrome P450 hydroxylase, CYP81A6, as the candidate for the bentazon and sulfonylurea herbicide resistance gene, Bel, in rice
Bentazon and sulfonylureas have been used for selective control of broadleaf weeds and sedges in rice fields for more than 20 years. A bentazon and sulfonylurea susceptible mutant, bel, was previously identified ...
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Article
Leaf epidermal characters of Lonicera japonica and Lonicera confuse and their ecology adaptation
The leaf epidermis of Japanese honeysuckle (Lonicera japonica Thunb.) and Wild Honeysuckle (Lonicera confusa) in the genus of Flos Lonicerae were mainly observed by scanning electron microscopes (SEM) to study th...
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Article
Homology priority task scheduling in μC/OS-II real-time kernel
μC/OS-II is an open source real-time kernel adopting priority preemptive schedule strategy. Aiming at the problem of μC/OS-II failing to support homology priority tasks scheduling, an approach for solution is ...
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Article
Induction, development and maturation of somatic embryos in Bunge’s pine (Pinus bungeana Zucc. ex Endl.)
Embryogenic tissue was induced from develo** immature zygotic embryos in Bunge’s pine (Pinus bungeana Zucc. ex Endl.). Induction rate reached 84.4% with our best treatment. Zygotic embryos were dissected from m...
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Detecting protein–protein interactions by far western blotting
Far western blotting (WB) was derived from the standard WB method to detect protein–protein interactions in vitro. In Far WB, proteins in a cell lysate containing prey proteins are firstly separated by SDS or nat...
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The effects of bioprocess parameters on extracellular proteases in a recombinant Aspergillus niger B1-D
Although host proteases are often considered to have a negative impact upon heterologous protein production by filamentous fungi, relatively little is known about the pattern of their appearance in recombinant...
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Article
Open AccessAberrant epigenetic changes and gene expression in cloned cattle dying around birth
Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneou...
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Open AccessPrevalence of A2143G mutation of H. pylori-23S rRNA in Chinese subjects with and without clarithromycin use history
A2143G mutation of 23S rRNA gene of H. pylori results in clarithromycin (CLR) resistance. To investigate the prevalence of the CLR resistance-related A2143G mutation of the H. pylori-specific 23S rRNA gene in Chi...
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Survey of damaging pests and preliminary assessment of forest health risks to the long term success of Pinus radiata introduction in Sichuan, southwest China
Pinus radiata was introduced to the summer rainfall environments of Sichuan Province, China in the 1990s as a part of an affore-station program for soil and water conservation in the arid and semi-arid river vall...
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Expression profiling analysis for genes related to meat quality and carcass traits during postnatal development of backfat in two pig breeds
The competitive equilibrium of fatty acid biosynthesis and oxidation in vivo determines porcine subcutaneous fat thickness (SFT) and intramuscular fat (IMF) content. Obese and lean-type pig breeds show obvious di...
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Perspectives of microbial oils for biodiesel production
Biodiesel has become more attractive recently because of its environmental benefits, and the fact that it is made from renewable resources. Generally speaking, biodiesel is prepared through transesterification...
