15 Result(s)
within
Pu Zheng
Microbiology
-
Article
Open AccessDevelopment of highly efficient and specific base editors in Actinobacillus succinogenes for enhancing succinic acid production
The production of bio-succinic acid (SA) from renewable feedstocks is a promising and sustainable approach to mitigating the high carbon emissions associated with the current energy crisis. Actinobacillus succino...
-
Article
Open AccessPurification and characterization of aspartic protease from Aspergillus niger and its efficient hydrolysis applications in soy protein degradation
Adding acid protease to feed can enhance protein digestibility, boost feed utilization, and stimulate the growth of animals in breading industry. In order to obtain an acid protease with high hydrolysis effici...
-
Article
Open AccessEffects of down-regulation of ackA expression by CRISPR-dCpf1 on succinic acid production in Actinobacillus succinogenes
Succinic acid (SA), a key intermediate in the cellular tricarboxylic acid cycle (TCA), is a 4-carbon dicarboxylic acid of great industrial value. Actinobacillus succinogenes can ferment various carbon sources and...
-
Article
Enhanced thermostability and catalytic efficiency of glucose oxidase in Pichia Pastoris
Glucose oxidase (GOD) has many practical applications, but its poor thermostability limits its broader use. In this research, three primary mutants of wild-type GOD were designed using rational mutagenesis, an...
-
Article
Effect of the Gad system on Actinobacillus succinogenes during acid stress
The glutamate decarboxylase (Gad) system is an important amino acid-dependent acid resistance system commonly found in microorganisms. Actinobacillus succinogenes is one of the best natural producers of succinic ...
-
Article
Construction of synthetic pathways for raspberry ketone production in engineered Escherichia coli
Raspberry ketone is an important ingredient in the flavor and fragrance industries. Due to its low content in fruits and vegetables, the production of natural raspberry ketone using heterologous synthesis in m...
-
Article
Muconic acid production from glucose using enterobactin precursors in Escherichia coli
Muconic acid (MA) is a promising bulk chemical due to its extensive industrial applications in the production of adipic acid and other valuable, biodegradable intermediates. MA is heretofore mainly produced fr...
-
Article
Enhanced succinic acid production by Actinobacillus succinogenes after genome shuffling
Succinic acid is an important platform chemical for synthesis of C4 compounds. We applied genome shuffling to improve fermentative production of succinic acid by A. succinogenes. Using a screening strategy compos...
-
Article
Spirit-based distillers’ grain as a promising raw material for succinic acid production
Spirit-based distillers’ grain (SDG) is the main by-product of the Chinese liquor industry, with an annual output of approx. 100 million tons. The economical potential of fermentative production of succinic ac...
-
Article
Genome shuffling improves thermotolerance and glutamic acid production of Corynebacteria glutamicum
Genome shuffling was used to improve the thermotolerance of l-glutamic acid-producing strain Corynebacteria glutamicum. Five strains with subtle improvements in high temperature tolerance and productivity were se...
-
Article
Display of α-amylase on the surface of Corynebacterium glutamicum cells by using NCgl1221 as the anchoring protein, and production of glutamate from starch
We developed a new cell surface display system in Corynebacterium glutamicum based on the C-terminally truncated NCgl1221 anchor protein to increase l-glutamate production from starch directly. The C-terminally t...
-
Article
Double deletion of dtsR1 and pyc induce efficient l-glutamate overproduction in Corynebacterium glutamicum
Corynebacterium glutamicum strains are used for the fermentative production of l-glutamate. Five C. glutamicum deletion mutants were isolated by two rounds of selection for homologous rec...
-
Article
Expression of Arginine Deiminase from Pseudomonas plecoglossicida CGMCC2039 in Escherichia coli and Its Anti-Tumor Activity
Arginine deiminase (ADI), an arginine-degrading enzyme, has been studied as a potential anti-cancer agent in clinical trials for the treatment of arginine-auxotrophic tumors, such as hepatocellular carcinomas ...
-
Article
Isolation and identification of an arginine deiminase producing strain Pseudomonas plecoglossicida CGMCC2039
Arginine deiminase (ADI), an arginine degrading enzyme, has been studied as a potential anti-cancer agent for arginine-auxotrophic tumors, such as melanomas and hepatocellular carcinomas (HCCs). In this study,...
-
Article
Biotransformation of Isoeugenol to Vanillin by a Novel Strain of Bacillus fusiformis
A novel strain of Bacillus fusiformis, producing high amounts of vanillin from isoeugenol, was isolated from soil. Using 60% (v/v) isoeugenol as substrate and solvent and at pH 4.0, 37 °C and 180 rpm, vanillin wa...
