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Article
Soft matter and nanomaterials characterization by cryogenic transmission electron microscopy
Soft matter has historically been an unlikely candidate for investigation by electron microscopy techniques due to damage by the electron beam as well as inherent instability under a high vacuum environment. C...
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Article
Open AccessTargeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor
G protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by mediating a GDP to GTP exchange in the Gα subunit. This leads to dissociation of the heterotrimer into Gα-GTP and Gβγ dimer. The Gα-GTP...
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Article
Open AccessA novel small molecule chaperone of rod opsin and its potential therapy for retinal degeneration
Rhodopsin homeostasis is tightly coupled to rod photoreceptor cell survival and vision. Mutations resulting in the misfolding of rhodopsin can lead to autosomal dominant retinitis pigmentosa (adRP), a progress...
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Article
Open AccessCryo-EM Visualization of Lipid and Polymer-Stabilized Perfluorocarbon Gas Nanobubbles - A Step Towards Nanobubble Mediated Drug Delivery
Gas microbubbles stabilized with lipids, surfactants, proteins and/or polymers are widely used clinically as ultrasound contrast agents. Because of their large 1–10 µm size, applications of microbubbles are co...
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Article
Open AccessBioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays
The 2014 Ebola epidemic is the largest to date. There is no cure or treatment for this deadly disease; therefore there is an urgent need to develop new diagnostics to accurately detect Ebola. Current RT-PCR as...
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Chapter
Adenovirus
Of the 53 different human adenovirus (HAdV) serotypes belonging to species A-G, a significant number are associated with acute respiratory, gastrointestinal and ocular infections. Replication-defective HAdV-5-...
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Chapter
Structural Aspects of the Cyanobacterial KaiABC Circadian Clock
The KaiABC circadian clock in the cyanobacterium Synechococcus elongatus can be reconstituted in vitro from three proteins in the presence of ATP. This oscillator displays the pertinent features of circadian rhyt...
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Article
Open AccessSea urchin vault structure, composition, and differential localization during development
Vaults are intriguing ribonucleoprotein assemblies with an unknown function that are conserved among higher eukaryotes. The Pacific coast sea urchin, Strongylocentrotus purpuratus, is an invertebrate model organi...
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Article
Lens α-crystallin: Function and structure
α-Crystallin is a major lens protein, comprising up to 40% of total lens proteins, where its structural function is to assist in maintaining the proper refractive index in the lens. In addition to its structur...
