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Article
Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro
In mammalian cells, DNA double-strand breaks are predominantly repaired by non-homologous end joining (NHEJ). During repair, the Ku70–Ku80 heterodimer (Ku), X-ray repair cross complementing 4 (XRCC4) in comple...
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Article
Small-molecule autocatalysis drives compartment growth, competition and reproduction
Sustained autocatalysis coupled to compartment growth and division is a key step in the origin of life, but an experimental demonstration of this phenomenon in an artificial system has previously proven elusiv...
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Article
Open AccessAdvances in single-scan time-encoding magnetic resonance imaging
Time-encoding MRI is a single-scan method that uses traditional k-encoding only in one direction. In the orthogonal “time-encoding” direction, a string of echoes appears in an order that depends on the position o...
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Article
Cross-correlation between a carbonyl C′ chemical shift anisotropy and a long-range dipolar C′HA coupling in proteins using symmetrical reconversion
A new sequence is described to measure the cross-correlation rates between the chemical shift anisotropy of the carbonyl carbon-13 nucleus and the dipole-dipole interaction between this carbonyl and the alpha-...
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Article
Correlated motions of successive amide N-H bonds in proteins
New nuclear magnetic resonance (NMR) methods are described for the measurement of cross-correlation rates of zero- and double-quantum coherences involving two nitrogen nuclei belonging to successive amino acid...
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Article
Simultaneous determination of Ψ and Φ angles in proteins from measurements of cross-correlated relaxation effects
A method is presented to determine both φ and ψ backbone angles in proteins simultaneously. This is achieved by measuring the effect on two-spin coherences of cross-correlation between 15 N-1HN and 13 ...
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Article
Efficient determination of angles subtended by Cα-Hα and N-HN vectors in proteins via dipole-dipole cross-correlation§
The angle ΘCαHα,NHN subtended by the internuclear vectors 13Cα-Hα and 15N-HN in doubly-labeled proteins can be determined by observing the effect of cross-correlation between the dipolar interactions on zero- ...
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Article
Normalized one-dimensional NOE measurements in isotopically labeled macromolecules using two-way cross-polarization
A novel one-dimensional NOE experiment is presented where a selected proton is excited by two-way heteronuclear cross- polarization between protons and nitrogen-15 or carbon-13. The utility of the method is de...
