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Article
Open AccessDisrupting Na+ ion homeostasis and Na+/K+ ATPase activity in breast cancer cells directly modulates glycolysis in vitro and in vivo
Glycolytic flux is regulated by the energy demands of the cell. Upregulated glycolysis in cancer cells may therefore result from increased demand for adenosine triphosphate (ATP), however it is unknown what th...
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Article
Open AccessModified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutations
Mechanosensitive channels are integral membrane proteins that sense mechanical stimuli. Like most plasma membrane ion channel proteins they must pass through biosynthetic quality control in the endoplasmic ret...
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Article
Open AccessSurface model of the human red blood cell simulating changes in membrane curvature under strain
We present mathematical simulations of shapes of red blood cells (RBCs) and their cytoskeleton when they are subjected to linear strain. The cell surface is described by a previously reported quartic equation ...
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Article
Open AccessEnhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy
We present the first direct nuclear magnetic resonance (NMR) evidence of enhanced entry of Ca2+ ions into human erythrocytes (red blood cells; RBCs), when these cells are mechanically distorted. For this we loade...
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Article
Open AccessRapid zero-trans kinetics of Cs+ exchange in human erythrocytes quantified by dissolution hyperpolarized 133Cs+ NMR spectroscopy
Transmembrane flux of Cs+ (a K+ congener) was measured in human red blood cells (RBCs; erythrocytes) on the 10-s time scale. This is the first report on dissolution dynamic nuclear polarization (dDNP) nuclear mag...
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Article
Open AccessGlyoxalase activity in human erythrocytes and mouse lymphoma, liver and brain probed with hyperpolarized 13C-methylglyoxal
Methylglyoxal is a faulty metabolite. It is a ubiquitous by-product of glucose and amino acid metabolism that spontaneously reacts with proximal amino groups in proteins and nucleic acids, leading to impairmen...
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Article
NMR magnetization-transfer analysis of rapid membrane transport in human erythrocytes
Nuclear magnetic resonance (NMR) magnetization-transfer (MT) experiments provide a convenient tool for studying rapid sub-second membrane-transport processes in situ in metabolically active cells. These experi...
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Article
Morphology and water permeability of red blood cells from green sea turtle (Chelonia mydas)
The morphology and diffusional water permeability (P d) of red blood cells (RBCs) from green sea turtle (GST) (Chelonia mydas) are presented for the first time. The RBCs had an ellipsoidal...
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Article
Membrane flickering of the human erythrocyte: physical and chemical effectors
Recent studies suggest a link between adenosine triphosphate (ATP) concentration and the amplitude of cell membrane flickering (CMF) in the human erythrocyte (red blood cell; RBC). Potentially, the origin of t...
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Article
Long-lived spin state of a tripeptide in stretched hydrogel
The longitudinal (T 1), transverse (T 2), and singlet state (T s) relaxation times of the geminal backbone protons (CH2) of l-Leu-Gly-Gly were studied by NMR spectr...
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Article
Membrane flickering of the human erythrocyte: constrained random walk used with Bayesian analysis
The involvement of adenosine triphosphate (ATP) in erythrocyte (red blood cell; RBC) membrane flickering is of particular interest, because ATP turnover in the cell as a whole is not yet fully accounted for. W...
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Article
Matrix-dependent modulation of anisotropic effects on NMR spectra from 7Li+ and 23Na+ encapsulated in cryptands
7Li and 23Na NMR spectra of the respective cations in gelatin and ι-carrageenan gels containing cryptand-[2.1.1] (for Li+) or cryptand-[2.2.2] (for Na+) displayed two transitions: the one at high...
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Article
Insights into hERG K+ channel structure and function from NMR studies
The unique gating kinetics of hERG K+ channels are critical for normal cardiac repolarization, and patients with mutations in hERG have a markedly increased risk of cardiac arrhythmias and sudden cardiac arrest. ...
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Article
NMR q-space analysis of canonical shapes of human erythrocytes: stomatocytes, discocytes, spherocytes and echinocytes
q-Space plots obtained experimentally using pulsed field-gradient stimulated echo (PGSTE) nuclear magnetic resonance (NMR) spectroscopy from water diffusing in red blood cells (RBCs) of different...
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Article
Detection of platypus-type l/d-peptide isomerase activity in aqueous extracts of papaya fruit
Peptide isomerase catalyses the post-translational isomerisation of the l- to the d-form of an amino acid residue around the N/C-termini of substrate peptides. To date, some peptide isomerases have been found in ...
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Article
Open AccessNovel venom gene discovery in the platypus
To date, few peptides in the complex mixture of platypus venom have been identified and sequenced, in part due to the limited amounts of platypus venom available to study. We have constructed and sequenced a c...
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Article
Mathematical Models of Naturally “Morphed” Human Erythrocytes: Stomatocytes and Echinocytes
We present two mathematical models that describe human red blood cells (RBCs) with morphologies that are attained naturally under certain patho-physiological conditions, namely stomatocytes and echinocytes. Mu...
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Article
Models of the human metabolic network: aiming to reconcile metabolomics and genomics
The metabolic syndrome, inborn errors of metabolism, and drug-induced changes to metabolic states all bring about a seemingly bewildering array of alterations in metabolite concentrations; these often occur in...
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Article
Proteins, membranes and cells: the structure–function nexus—ASB 2008
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Article
Structure of the pore-helix of the hERG K+ channel
The hERG K+ channel undergoes rapid inactivation that is mediated by ‘collapse’ of the selectivity filter, thereby preventing ion conduction. Previous studies have suggested that the pore-helix of hERG may be up ...
