22 Result(s)
within
Philip W Kuchel
Cell Biology
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Article
Open AccessDisrupting Na+ ion homeostasis and Na+/K+ ATPase activity in breast cancer cells directly modulates glycolysis in vitro and in vivo
Glycolytic flux is regulated by the energy demands of the cell. Upregulated glycolysis in cancer cells may therefore result from increased demand for adenosine triphosphate (ATP), however it is unknown what th...
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Article
NMR magnetization-transfer analysis of rapid membrane transport in human erythrocytes
Nuclear magnetic resonance (NMR) magnetization-transfer (MT) experiments provide a convenient tool for studying rapid sub-second membrane-transport processes in situ in metabolically active cells. These experi...
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Article
Morphology and water permeability of red blood cells from green sea turtle (Chelonia mydas)
The morphology and diffusional water permeability (P d) of red blood cells (RBCs) from green sea turtle (GST) (Chelonia mydas) are presented for the first time. The RBCs had an ellipsoidal...
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Article
Membrane flickering of the human erythrocyte: physical and chemical effectors
Recent studies suggest a link between adenosine triphosphate (ATP) concentration and the amplitude of cell membrane flickering (CMF) in the human erythrocyte (red blood cell; RBC). Potentially, the origin of t...
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Article
Membrane flickering of the human erythrocyte: constrained random walk used with Bayesian analysis
The involvement of adenosine triphosphate (ATP) in erythrocyte (red blood cell; RBC) membrane flickering is of particular interest, because ATP turnover in the cell as a whole is not yet fully accounted for. W...
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Article
Matrix-dependent modulation of anisotropic effects on NMR spectra from 7Li+ and 23Na+ encapsulated in cryptands
7Li and 23Na NMR spectra of the respective cations in gelatin and ι-carrageenan gels containing cryptand-[2.1.1] (for Li+) or cryptand-[2.2.2] (for Na+) displayed two transitions: the one at high...
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Article
Insights into hERG K+ channel structure and function from NMR studies
The unique gating kinetics of hERG K+ channels are critical for normal cardiac repolarization, and patients with mutations in hERG have a markedly increased risk of cardiac arrhythmias and sudden cardiac arrest. ...
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Article
NMR q-space analysis of canonical shapes of human erythrocytes: stomatocytes, discocytes, spherocytes and echinocytes
q-Space plots obtained experimentally using pulsed field-gradient stimulated echo (PGSTE) nuclear magnetic resonance (NMR) spectroscopy from water diffusing in red blood cells (RBCs) of different...
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Article
Mathematical Models of Naturally “Morphed” Human Erythrocytes: Stomatocytes and Echinocytes
We present two mathematical models that describe human red blood cells (RBCs) with morphologies that are attained naturally under certain patho-physiological conditions, namely stomatocytes and echinocytes. Mu...
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Article
Proteins, membranes and cells: the structure–function nexus—ASB 2008
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Article
Structure of the pore-helix of the hERG K+ channel
The hERG K+ channel undergoes rapid inactivation that is mediated by ‘collapse’ of the selectivity filter, thereby preventing ion conduction. Previous studies have suggested that the pore-helix of hERG may be up ...
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Article
Erythrocyte orientational and cell volume effects on NMR q-space analysis: simulations of restricted diffusion
Pulsed field-gradient spin echo nuclear magnetic resonance spectroscopy of water diffusing in erythrocytes leads to diffusion interference and diffraction effects, which are visualised in q-space plots of signal ...
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Article
Effectors of the frequency of calcium oscillations in HEK-293 cells: wavelet analysis and a computer model
Oscillations of the intracellular concentration of Ca2+ in cultured HEK-293 cells, which heterologously expressed the calcium-sensing receptor, were recorded with the fluorophore Fura-2 using fluorescence microsc...
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Article
Human erythrocyte flickering: temperature, ATP concentration, water transport, and cell aging, plus a computer simulation
Images of human erythrocytes from a healthy donor were recorded under differential interference contrast (DIC) microscopy; they were acquired rapidly (~336 Hz) and the intensity of the centermost pixel of each...
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Article
39K nuclear magnetic resonance and a mathematical model of K+ transport in human erythrocytes
39K nuclear magnetic resonance was used to measure the efflux of K+ from suspensions of human erythrocytes [red blood cells (RBCs)], that occurred in response to the calcium ionophore, A23187 and calcium ions; th...
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Article
The HERG K+ channel: progress in understanding the molecular basis of its unusual gating kinetics
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Article
The HERG K+ channel: progress in understanding the molecular basis of its unusual gating kinetics
The HERG K+ channel has very unusual kinetic behaviour that includes slow activation but rapid inactivation. These features are critical for normal cardiac repolarisation as well as in preventing lethal ventricul...
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Article
Simulations of NMR-detected diffusion in suspensions of red cells: the effects of variation in membrane permeability and observation time
Monte Carlo random-walk simulations of diffusion in virtual lattices of cells have been used to study and characterize diffusion-coherence phenomena that arise when pulsed field-gradient spin-echo (PGSE) nucle...
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Article
Simulations of NMR-detected diffusion in suspensions of red cells: the "signatures" in q-space plots of various lattice arrangements
Coherence effects from pulsed field-gradient spin-echo (PGSE) nuclear magnetic resonance diffusion experiments have been observed and characterized for diffusants in many heterogeneous systems, ranging from p...
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Article
1H NMR of compounds with low water solubility in the presence of erythrocytes: effects of emulsion phase separation
When lipophilic compounds like diethyl phthalate (DEP) were added to water, two sets of resonances appeared in the 1H NMR spectrum, whereas when added in concentrations above ~3.5 mM to erythrocytes in a high ha...
