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Protocol
Analysis of Phase Separation of EARLY FLOWERING 3
Phase separation is an important mechanism for regulating various cellular functions. The EARLY FLOWERING 3 (ELF3) protein, an essential element of the EVENING COMPLEX (EC) involved in circadian clock regulati...
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Protocol
Monitoring Real-Time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism
RNA molecules play crucial roles in gene expression regulation and cellular signaling, and these functions are governed by the formation of RNA secondary and tertiary structures. These structures are highly dy...
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Protocol
In Vitro Determination of Temperature-Dependent DNA Binding of the Evening Complex Using Electrophoretic Mobility Shift Assays
Electrophoretic mobility shift assays (EMSAs) of DNA-binding proteins and labeled DNA allow the qualitative and quantitative characterization of protein-DNA complex formation using native (nondenaturing) polya...
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Article
Open AccessPhytochromes transmit photoperiod information via the evening complex in Brachypodium
Daylength is a key seasonal cue for animals and plants. In cereals, photoperiodic responses are a major adaptive trait, and alleles of clock genes such as PHOTOPERIOD1 (PPD1) and EARLY FLOWERING3 (ELF3) have been...
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Article
Correction to: ChIP-seq and RNA-seq for complex and low-abundance tree buds reveal chromatin and expression co-dynamics during sweet cherry bud dormancy
The correct name of the 3rd Author is presented in this paper.
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Article
A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis
Temperature controls plant growth and development, and climate change has already altered the phenology of wild plants and crops1. However, the mechanisms by which plants sense temperature are not well understood...
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Article
An RNA thermoswitch regulates daytime growth in Arabidopsis
Temperature is a major environmental cue affecting plant growth and development. Plants often experience higher temperatures in the context of a 24 h day–night cycle, with temperatures peaking in the middle of...
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Article
Open AccessChIP-seq and RNA-seq for complex and low-abundance tree buds reveal chromatin and expression co-dynamics during sweet cherry bud dormancy
Chromatin immunoprecipitation-sequencing (ChIP-seq) is a robust technique to study interactions between proteins, such as histones or transcription factors and DNA. This technique in combination with RNA-seque...
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Article
Open AccessFrom bud formation to flowering: transcriptomic state defines the cherry developmental phases of sweet cherry bud dormancy
Bud dormancy is a crucial stage in perennial trees and allows survival over winter to ensure optimal flowering and fruit production. Recent work highlighted physiological and molecular events occurring during ...
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Protocol
Chromatin Immunoprecipitation Sequencing (ChIP-Seq) for Transcription Factors and Chromatin Factors in Arabidopsis thaliana Roots: From Material Collection to Data Analysis
Chromatin immunoprecipitation combined with next-generation sequencing (ChIP-seq) is a powerful technique to investigate in vivo transcription factor (TF) binding to DNA, as well as chromatin marks. Here we pr...
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Article
The evening complex coordinates environmental and endogenous signals in Arabidopsis
Plants maximize their fitness by adjusting their growth and development in response to signals such as light and temperature. The circadian clock provides a mechanism for plants to anticipate events such as su...
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Article
Open AccessTradict enables accurate prediction of eukaryotic transcriptional states from 100 marker genes
Transcript levels are a critical determinant of the proteome and hence cellular function. Because the transcriptome is an outcome of the interactions between genes and their products, it may be accurately repr...
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Article
Molecular and genetic control of plant thermomorphogenesis
Temperature is a major factor governing the distribution and seasonal behaviour of plants. Being sessile, plants are highly responsive to small differences in temperature and adjust their growth and developmen...
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Article
Open AccessThermal stress effects on grain yield in Brachypodium distachyon occur via H2A.Z-nucleosomes
Crop plants are highly sensitive to ambient temperature, with a 1 ºC difference in temperature sufficient to affect development and yield. Monocot crop plants are particularly vulnerable to higher temperatures...
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Article
Transcription factor PIF4 controls the thermosensory activation of flowering
A novel mechanism by which warming temperatures can directly activate flowering in the model plant Arabidopsis thaliana.
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Article
Florigen takes two to tango
A recent flurry of activity has defined the protein FLOWERING LOCUS T (FT) as a mobile signal or 'florigen' that induces flowering. A new study has shown that a 14-3-3 protein links FT to a transcription facto...
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Article
Open AccessThe dynamic genome of Hydra
Hydra, first described by Anton van Leeuwenhoek in a letter to the Royal Society in 1702, has been studied by biologists for centuries and now is an important model for work on axial patterning, stem cell biology...
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Article
Red sky in the morning, shepherd's warning
A new study provides insight into the way plants integrate information from the environment to anticipate the onset of winter. Plants are able to combine information about light quality and ambient temperature...