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Article
Open AccessFluorescent molecular rotors as versatile in situ sensors for protein quantitation
Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of as...
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Article
Open AccessTranscriptomic analysis of a 3D blood–brain barrier model exposed to disturbed fluid flow
Cerebral aneurysms are more likely to form at bifurcations in the vasculature, where disturbed fluid is prevalent due to flow separation at sufficiently high Reynolds numbers. While previous studies have demon...
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Article
Open AccessRecombinant human plasma gelsolin reverses increased permeability of the blood–brain barrier induced by the spike protein of the SARS-CoV-2 virus
Plasma gelsolin (pGSN) is an important part of the blood actin buffer that prevents negative consequences of possible F-actin deposition in the microcirculation and has various functions during host immune res...
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Article
SARS-CoV-2 Spike Protein Disrupts Blood–Brain Barrier Integrity via RhoA Activation
The SARS-CoV-2 spike protein has been shown to disrupt blood–brain barrier (BBB) function, but its pathogenic mechanism of action is unknown. Whether angiotensin converting enzyme 2 (ACE2), the viral binding s...
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Article
Open AccessPeristaltic flow in the glymphatic system
The flow inside the perivascular space (PVS) is modeled using a first-principles approach in order to investigate how the cerebrospinal fluid (CSF) enters the brain through a permeable layer of glial cells. Lu...
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Article
Open AccessHarnessing neurovascular interaction to guide axon growth
Regulating the intrinsic interactions between blood vessels and nerve cells has the potential to enhance repair and regeneration of the central nervous system. Here, we evaluate the efficacy of aligned microve...
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Article
Open AccessUncoupling shear and uniaxial elastic moduli of semiflexible biopolymer networks: compression-softening and stretch-stiffening
Gels formed by semiflexible filaments such as most biopolymers exhibit non-linear behavior in their response to shear deformation, e.g., with a pronounced strain stiffening and negative normal stress. These ne...
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Article
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Tissues with perfusable vascular networks can be fabricated through layer-by-layer assembly, bioprinting or sacrificial moulding, but current approaches are slow, have limited resolution, or place significant ...
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Article
A Linear, Biphasic Model Incorporating a Brinkman Term to Describe the Mechanics of Cell-Seeded Collagen Hydrogels
Protein-based hydrogels are commonly used as in vitro models of native tissues because they can mimic specific aspects of the three-dimensional extracellular matrix present in vivo. Bulk mechanical stimulation is...
