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Protocol
Distribution of Small RNAs Along Transposable Elements in Vitis vinifera During Somatic Embryogenesis
Metaviridae is a family of reverse-transcribing viruses, closely related to retroviruses; they exist within their host’s DNA as transposable elements. Transposable element study requires the use of specialized to...
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Protocol
Detection of Pathogenesis-Related (PR) Genes in Susceptible Tomato Plants Infected by Meloidogyne spp.
Meloidogyne species, as infective second-stage juveniles (J2s) larvae, are parasites able to attack host of relevant agronomic interest such as tomato plants. The identification of gene expression markers, use...
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Article
Open AccessExogenous and endogenous dsRNAs perceived by plant Dicer-like 4 protein in the RNAi-depleted cellular context
In plants, RNase III Dicer-like proteins (DCLs) act as sensors of dsRNAs and process them into short 21- to 24-nucleotide (nt) (s)RNAs. Plant DCL4 is involved in the biogenesis of either functional endogenous ...
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Article
Open AccessRegulation of plant antiviral defense genes via host RNA-silencing mechanisms
Plants in nature or crops in the field interact with a multitude of beneficial or parasitic organisms, including bacteria, fungi and viruses. Viruses are highly specialized to infect a limited range of host pl...
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Protocol
ROS and Cell Death in Tomato Roots Infected by Meloidogyne Incognita
Phytoparasitic nematodes are plant pests causing serious problems to a broad range of hosts, and Meloidogyne species are widely recognized as the most damaging among the root knot nematode groups. During the inco...
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Article
Host-suitability of black medick (Medicago lupulina L.) and additional molecular markers for identification of the pea cyst nematode Heterodera goettingiana
A survey was conducted in 16 fields cultivated with broad bean (Vicia faba L.) and garden pea (Pisum sativum L.) in nine localities of Apulia, southern Italy, to determine whether annual weeds were susceptible to...
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Article
Systems biology and genome-wide approaches to unveil the molecular players involved in the pre-germinative metabolism: implications on seed technology traits
The pre-germinative metabolism is among the most fascinating aspects of seed biology. The early seed germination phase, or pre-germination, is characterized by rapid water uptake (imbibition), which directs a ...
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Article
Induction of SA-signaling pathway and ethylene biosynthesis in Trichoderma harzianum-treated tomato plants after infection of the root-knot nematode Meloidogyne incognita
Salicylic acid-signaling pathway and ethylene biosynthesis were induced in tomato treated with Trichoderma harzianum when infected by root-knot nematodes and limited the infection by activation of SAR and ethylen...
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Chapter
The Influence of Phosphate Deficiency on Legume Symbiotic N2 Fixation
Legumes are well recognized for their nutritional and health benefits as well as for their impact in the sustainability of agricultural systems. Phosphate (Pi) deficiency is a major nutritional factor limiting...
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Article
Parasitism effects on white clover by root-knot and cyst nematodes and molecular separation of Heterodera daverti from H. trifolii
This research carried out an accurate identification of the root-knot and cyst-forming nematode species parasitizing white clover at the Laceno Lake area in Southern Italy. Two species, the root-knot nematode Mel...
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Article
Benzothiadiazole effect in the compatible tomato-Meloidogyne incognita interaction: changes in giant cell development and priming of two root anionic peroxidases
BTH application is effective in root-knot nematode-tomato interaction in a way that involves a delay in the formation of nematode feeding site and triggers molecular responses at several levels...
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Article
Nematicidal potential of Artemisia annua and its main metabolites
Nematotoxic effect of an aqueous extract of Artemisia annua and its components caffeic acid, chlorogenic acid (5-caffeoylquinic acid, 5-CQA), artemisinin and the related semi-synthetic artesunate, was investigate...
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Article
ROS and NO production in compatible and incompatible tomato-Meloidogyne incognita interactions
Nitric oxide (NO) has been shown to be an essential regulatory molecule in plant response to pathogen infection in synergy with reactive oxygen species (ROS). At the present, nothing is known about the role of...
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Article
Open AccessGenome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita
Biological control of the root-knot nematode Meloidogyne incognita, one of the world's most destructive crop pathogens, presents a major opportunity for safely improving global agricultural yields. Its 86-Mb geno...
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Article
Correlation Between Decreased Expression of Mitochondrial F0F1-ATP Synthase and Low Regenerating Capability of the Liver after Partial Hepatectomy in Hypothyroid Rats
In hypothyroid rats, partial hepatectomy does not induce liver regeneration until 120 h aftersurgical operation, when, instead, in normal rats a complete recovery of the liver mass, in thisinterval, is observe...
