-
Article
Inheritance and map** of the ore gene controlling the quantity of β-carotene in cucumber (Cucumis sativus L.) endocarp
The metabolic precursor of vitamin A, β-carotene, is essential for human health. The gene(s) controlling β-carotene quantity (QβC) has been introgressed from **shuangbanna gourd (XIS, possessing β-carotene; Cucum...
-
Article
Mining and validation of pyrosequenced simple sequence repeats (SSRs) from American cranberry (Vaccinium macrocarpon Ait.)
The American cranberry (Vaccinium macrocarpon Ait.) is a major commercial fruit crop in North America, but limited genetic resources have been developed for the species. Furthermore, the paucity of codominant DNA...
-
Article
Inheritance of beta-carotene-associated mesocarp color and fruit maturity of melon (Cucumis melo L.)
Melon (Cucumis melo L.) fruit production in U.S. can be improved through the introgression of early fruit maturity (FM) and the enhancement of fruit color [i.e., quantity of β-carotene (QβC); orange mesocarp]. Ho...
-
Article
Inheritance of beta-carotene-associated flesh color in cucumber (Cucumis sativus L.) fruit
The nutritional value of cucumber (Cucumis sativus L.) can be improved by the introgression of β-carotene (i.e., provitamin A and/or orange flesh) genes from “**shuangbanna gourd” (XIS; Cucumis sativus var. xishu...
-
Article
A consensus linkage map identifies genomic regions controlling fruit maturity and beta-carotene-associated flesh color in melon (Cucumis melo L.)
The nutritional value and yield potential of US Western Ship** melon (USWS; Cucumis melo L.) could be improved through the introgression of genes for early fruit maturity (FM) and the enhancement of the quantit...
-
Article
Map** of genetic loci that regulate quantity of beta-carotene in fruit of US Western Ship** melon (Cucumis melo L.)
Melon (Cucumis melo L.) is highly nutritious vegetable species and an important source of β-carotene (Vitamin A), which is an important nutrient in the human diet. A previously developed set of 81 recombinant inb...
-
Article
Geographical distribution of wild Daucus species in the natural grasslands of the Argentinian pampas
Three wild carrot species have been reported in the argentinian flora: Daucus montanus Humb. et Bonpl. ex Schult., D. montevidensis Link ex Sprengel and D. pusillus Michx. There is a discrepancy among authors abo...
-
Article
Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome map**
Carotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin A precursors α- and β-carotene. Carotenoids play essential biological roles in plants and ...
-
Article
Demonstration of linkage and development of the first low-density genetic map of garlic, based on AFLP markers
Garlic (Allium sativum L.) is a long-cultivated, clonally propagated diploid plant (2n=2x=16). With routine seed production now underway, we used populations (MP1 and MP2) generated by self-pollination of unrelat...
-
Article
Lycopene and β-carotene are bioavailable from lycopene ‘red’ carrots in humans
Objective: The purpose of this study was to determine if lycopene and β-carotene are bioavailable from lycopene red carrots and if lycopene absorption is affected by carrot fiber.
-
Article
Employment of flanking codominant STS markers to estimate allelic substitution effects of a nematode resistance locus in carrot
In carrot, two codominant sequence-tagged site (STS) markers, flanking in tight linkage the Meloidogyne javanica resistance (Mj-1) locus, were employed to investigate the association between expression of resista...
-
Article
Modified protocols for rapid carrot genomic DNA extraction and AFLP™ analysis using silver stain or radioisotopes
AFLP™ is one of the most frequently used techniques for identification of molecular markers. We have modified the procedures for genomic DNA extraction, AFLP product generation and silver staining in order to spe...
-
Article
Evidence for simply inherited dominant resistance to Meloidogyne javanica in carrot
Root-knot nematodes (Meloidogyne spp.) are serious pests of carrot (Daucus carota L.) worldwide. While soil treatment with nematicides is the primary means for managing nematodes in carrot, there is a need to ide...
-
Article
RAPD linkage map of the genomic region encompassing the root-knot nematode (Meloidogyne javanica) resistance locus in carrot
Inheritance studies have indicated that resistance to the root-knot nematode (Meloidogyne javanica) in carrot inbred line ’Brasilia-1252’ is controlled by the action of one or two (duplicated) dominant gene(s) lo...
-
Article
Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time
A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 μM 2,4-D and maintained on the...
-
Article
Linkage relationships among molecular markers and storage root traits of carrot (Daucus carota L. ssp. sativus)
A 109-point linkage map consisting of three phenotypic loci (P 1, Y 2, and Rs), six restriction fragment length polymorphisms (RFLPs), two random amplified polymorphic DNAs (RAPDs), 96 amplified fragment length ...
-
Article
Evidence for maternal inheritance of the chloroplast genome in cultivated carrot (Daucus carota L. ssp. sativus)
The incidence and inheritance of a chloroplast DNA (cpDNA) mutation/marker, BP10U, was studied in crosses among cultivated carrots (Daucus carota ssp. sativus). BP10U is about 400 bp larger than the more common ...
-
Article
Conversion of an AFLP fragment linked to the carrot Y2 locus to a simple, codominant,]PCR-based marker form
Recent advances have expanded the potential usefulness of molecular techniques for plant genetic research. AFLP is a powerful technique, allowing rapid and reliable analysis of multiple, potentially polymorph...
-
Article
Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets
Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones `DDR7099', `PI383819', and `Piacenza' were utilized as an explant source for continuous, friable callus production. The bes...
-
Article
True seed production in garlic
Despite a long history of obligate vegetative propagation, selected garlic clones can produce sexual seeds. By removing vegetative topsets from the inflorescence and cutting inflorescences from the underground...