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Chapter
Two-Dimensional Thin Layer Chromatography. Separation of Lipids Extracted from Native and Phospholipase A2 Treated Human Erythrocyte Ghosts
Qualitative and quantitative analyses of (phospho)lipids play an important role in studies on the structure and function of biological membranes. Even the investigator who is primarily interested in the protei...
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Chapter
Purification by Affinity Chromatography of Red Cell Membrane Acetylcholinesterase
Over the past the methods for purifying integral membrane proteins have advanced considerably. In particular the onset of affinity chromatography (Jakoby and Wilchek 1974) has brought new incentives to this pa...
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Chapter
Preparation of Spectrin-Free Vesicles from Human Red Blood Cells
An important prerequisite in the study of erythrocyte membrane structure and function is the availability of suitable membrane preparations. Several methods by which such “ghosts” can be prepared have been pub...