32 Result(s)

Article

A common mechanism for the mechanosensitive regulation of apoptosis in different cell types and for different mechanical stimuli

In all kinds of tissue cells are influenced by mechanical forces. In vivo fibroblasts are exposed to mechanical tension and endothelial cells are subjected directly to hemodynamic flow. It has been shown that dis...

R. Graf, S. Apenberg, M. Freyberg, P. Friedl in Apoptosis (2003)

Article

Mechanosensitive induction of apoptosis in fibroblasts is regulated by thrombospondin-1 and integrin associated protein (CD47)

Fibroblasts are cultured in three-dimensional collagen matrices to investigate the effect of mechanical tension on the regulation of apoptosis. Under the influence of mechanical loading, the cells show little ...

R. Graf, M. Freyberg, D. Kaiser, P. Friedl in Apoptosis (2002)

Article

The role of thrombospondin-1 in apoptosis

The thrombospondins are a family of extracellular proteins that participate in cell-to-cell and cell-to-matrix communication. They regulate cellular phenotype during tissue genesis and repair. Five family mem...

P. Friedl, P. Vischer, M. A. Freyberg in Cellular and Molecular Life Sciences CMLS (2002)

Chapter

Development and Validation of an Image Analysis System for Single Cell Characterization in Cell Monolayers

The results received either by counting via eye sight or by the use of the image analysis system coincided very well as demonstrated with the determination of the apoptotic and proliferative index of an endoth...

D. Kaiser, M. A. Freyberg, G. von Wichert… in New Developments and New Applications in A… (2002)

Chapter

Development of an Optically Accessible Perfusion Chamber for In Situ Assays and for Long-term Cultivation of Mammalian Cells

We have developed a perfusion system on the basis of normal cell culture plates. The cultivation of various cell lines is possible under more physiological conditions than in static culture. Futhermore adheren...

M. A. Freyberg… in New Developments and New Applications in Animal Cell Technology (2002)

Article

Vascular endothelial cells express a functional fas-receptor due to lack of hemodynamic forces

The fas system is present in atherosclerotic lesions. However, its role in the initiation and progression is still unclear. Here we show that in endothelial cells (EC) the expression of the fas receptor is reg...

M.-A. Freyberg, D. Kaiser, R. Graf, P. Friedl in Apoptosis (2001)

Article

The biology of cell locomotion within three-dimensional extracellular matrix

Cell migration in three-dimensional (3-D) extracellular matrix (ECM) is not a uniform event but rather comprises a modular spectrum of interdependent biophysical and biochemical cell functions. Haptokinetic c...

P. Friedl, E.-B. Bröcker in Cellular and Molecular Life Sciences CMLS (2000)

Article

The use of a conventional tissue culture plate as an optically accessible perfusion chamber for in situ assays and for long-term cultivation of mammalian cells

An alternative culture system has been developed based on a conventional tissue culture plate (3.5 cm diameter) which is changed into a closed perfusion chamber. The system can easily be scaled up from one to ...

M. A. Freyberg, P. Friedl in Cytotechnology (1998)

Chapter

Distinct Dynamics and Regulatory Signal Transduction of Cell Migration

In the adult metazoan organism active migration is an exclusive property of a few specialized cells. In the human body, cells of the immune system are highly active migratory cells. The ability of flexible pos...

Dr. F. Entschladen, K. Maaser, M. Gunzer, P. Friedl, B. Niggemann… in The Biology of Tumors (1998)

Chapter and Conference Paper

Konfrontation humaner T-Lymphozyten mit autologen Tumorexplantaten in 3-D Kollagenmatrices in vitro

Eine erfolgreiche T-Zell-vermittelte zytotoxische Anti-Tumor-Immunreaktion erfordert mehrere aufeinander koordinierte Effektorfunktionen der T-Zelle:

1. ...

P. Friedl, B. Niggemann, E.-B. Bröcker, K. S. Zänker in Dermatologie (1998)

Article

Effect of a mistletoe extract (Iscador® QuFrF) on viability and migratory behavior of human peripheral CD4⁺ and CD8⁺ T lymphocytes in three-dimensional collagen lattices

Migration and tissue distribution of immunocompetent cells may be critical prerequisites for efficient immune surveillance. The effect of various concentrations of the mistletoe extract Iscador® QuFrF on the l...

G. Nikolai, P. Friedl, M. Werner… in In Vitro Cellular & Developmental Biology … (1997)

Chapter

Metabolism of Confluent Vascular Endothelial Cell Cultures

Confluent endothelial monolayers were kept for a period of about 75 h without medium change. The concentrations of D-glucose, L-lactate, ammonium ions and amino acids in the conditioned media were determined a...

G. Schrimpf, M. Schröder, E. Weitnauer… in Animal Cell Technology: Developments Towar… (1995)

Article

Invasion and migration of coordinated cell clusters in rhabdomyosarcoma and other head and neck tumors in a 3-D collagen matrix

P. Friedl, P. B. Noble, P. A. Walton… in Journal of Cancer Research and Clinical On… (1995)

Article

Metabolic rates of vascular endothelial cellsin vitro

Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients — amino aci...

G. Schrimpf, M. Schröder, E. Weitnauer, P. Friedl in Cytotechnology (1994)

Article

Growth of human vascular endothelial cells on various types of microcarriers

Various types of microcarriers were tested as growth substrate for the cultivation of either endothelial cells from human umbilical cord veins or of EA. hy926, an immortalized cell line of endothelial origin. ...

G. Schrimpf, P. Friedl in Cytotechnology (1993)

Article

Palliation of esophageal cancer — operative resection versus laser and afterloading therapy

Since 1985, 71 patients with end-stage esophageal cancer have been treated either by surgical (n=26) or endoscopic laser palliation (n=45). In 16 of 45 patients treated by endoscopy, additional radiotherapy (extr...

T. Hölting, P. Friedl, N. Schraube, P. Fritz, P. Schlag, C. Herfarth in Surgical Endoscopy (1991)

Article

Tierexperimentelle untersuchungen zur toxizität von fluordesoxyuridin (FUDR) und 5-fluorouracil (FU) im rahmen der regionalen leberinfusionschemotherapie

In this study we examined the cytostatic compounds 5 FU and FUDR which are most frequently used in regional chemotherapy for any incidence of hepatobiliary toxicity in animals. For this we compared the intraar...

P. Schlag, J. Yi, P. Friedl, W. Hull, M. Berger in Langenbecks Archiv für Chirurgie (1990)