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    Acid polysaccharide content of frog rod outer segments determined by metachromatic toluidine blue staining

    Sulfonation of periodate-oxidized vicinal hydroxyl groups on a polysaccharide backbone allows binding of toluidine blue (aldehyde bisulfite-toluidine blue or ABT staining) with a concurrent metachromatic shift...

    R. E. Drzymala, P. A. Liebman, G. Romhanyi in Histochemistry (1982)