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Article
Contributions of lignification, tissue arrangement patterns, and cross-sectional area to whole-stem mechanical properties in Arabidopsis thaliana
Plant cells withstand mechanical stress originating from turgor pressure by robustly maintaining the mechanical properties of the cell wall. This applies at the organ scale as well; many plant stems act as pre...
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Article
Why is FERONIA pleiotropic?
The plant cell wall has many roles: structure, hydraulics, signalling and immunity. Monitoring its status is therefore essential for plant life. Among many candidate cell wall sensors, FERONIA, a member of the Ca...
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Protocol
Single-Cell Confinement Methods to Study Plant Cytoskeleton
Progress in cytoskeletal research in animal systems has been accompanied by the development of single-cell systems (e.g., fibroblasts in culture). Single-cell systems exist for plant research, but the presence...
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Article
Inducible depletion of PI(4,5)P2 by the synthetic iDePP system in Arabidopsis
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a low-abundance membrane lipid essential for plasma membrane function1,2. In plants, mutations in phosphatidylinositol 4-phosphate (PI4P) 5-kinases (PIP5K) sug...
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Article
Plant scientists can’t ignore Jevons paradox anymore
Although the ‘rebound effect’ is well established in environmental economics, the sometimes paradoxical effects of yield increase are rarely questioned within the plant science community. Acknowledging the cur...
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Article
Robust organ size requires robust timing of initiation orchestrated by focused auxin and cytokinin signalling
Organ size and shape are precisely regulated to ensure proper function. The four sepals in each Arabidopsis thaliana flower must maintain the same size throughout their growth to continuously enclose and protect ...
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Article
Open AccessMicrotubule self-organisation during seed germination in Arabidopsis
Upon water uptake and release of seed dormancy, embryonic plant cells expand, while being mechanically constrained by the seed coat. Cortical microtubules (CMTs) are key players of cell elongation in plants: t...
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Article
Open AccessAre microtubules tension sensors?
Mechanical signals play many roles in cell and developmental biology. Several mechanotransduction pathways have been uncovered, but the mechanisms identified so far only address the perception of stress intens...
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Article
Open AccessImageJ SurfCut: a user-friendly pipeline for high-throughput extraction of cell contours from 3D image stacks
Many methods have been developed to quantify cell shape in 2D in tissues. For instance, the analysis of epithelial cells in Drosophila embryogenesis or jigsaw puzzle-shaped pavement cells in plant epidermis ha...
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Protocol
Time-Lapse Imaging of Develo** Shoot Meristems Using A Confocal Laser Scanning Microscope
Analysis of meristem shape and gene expression pattern has been conducted in many species over the past decades. Recent live imaging techniques have allowed for an unprecedented accumulation of data on the bio...
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Article
Open AccessA phosphoinositide map at the shoot apical meristem in Arabidopsis thaliana
In plants, the shoot apical meristem (SAM) has two main functions, involving the production of all aerial organs on the one hand and self-maintenance on the other, allowing the production of organs during the ...
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Chapter
Mechanical Conflicts in Growth Heterogeneity
Morphogenesis involves coordinated cell division and cellular growth. Beyond average growth rate and direction, multicellular growth can also be characterized by its variance, i.e., the level of heterogeneity ...
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Article
Open AccessA mechanosensitive Ca2+ channel activity is dependent on the developmental regulator DEK1
Responses of cells to mechanical stress are thought to be critical in coordinating growth and development. Consistent with this idea, mechanically activated channels play important roles in animal development....
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Article
Open AccessNuclear envelope: a new frontier in plant mechanosensing?
In animals, it is now well established that forces applied at the cell surface are propagated through the cytoskeleton to the nucleus, leading to deformations of the nuclear structure and, potentially, to modi...
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Article
Open AccessLife behind the wall: sensing mechanical cues in plants
There is increasing evidence that all cells sense mechanical forces in order to perform their functions. In animals, mechanotransduction has been studied during the establishment of cell polarity, fate, and di...
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Article
FibrilTool, an ImageJ plug-in to quantify fibrillar structures in raw microscopy images
Cell biology heavily relies on the behavior of fibrillar structures, such as the cytoskeleton, yet the analysis of their behavior in tissues often remains qualitative. Image analysis tools have been developed ...
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Protocol
Time-Lapse Imaging of Develo** Meristems Using Confocal Laser Scanning Microscope
Analysis of shoot meristem shape and gene expression pattern has been conducted in many species over the past decades. Recent live imaging techniques have allowed an unprecedented accumulation of data on the b...
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Article
Organogenesis from stem cells in planta: multiple feedback loops integrating molecular and mechanical signals
In multicellular organisms, the coordination of cell behaviors largely relies on biochemical and biophysical signals. Understanding how such signals control development is often challenging, because their dist...
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Article
Open AccessQuantitative imaging strategies pave the way for testable biological concepts
In developmental biology, the accumulation of qualitative phenotypic descriptions has fueled the need for testable parsimonious hypotheses, giving a fresh impetus to quantitative strategies. As an illustration...
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Chapter and Conference Paper
Statistical Properties of Cell Topology and Geometry in a Tissue-Growth Model
Statistical properties of cell topologies in two-dimensional tissues have recently been suggested to be a consequence of cell divisions. Different rules for the positioning of new walls in plants have been pro...
