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Article
Gene expression and alternative splicing contribute to adaptive divergence of ecotypes
Regulation of gene expression is a critical link between genotype and phenotype explaining substantial heritable variation within species. However, we are only beginning to understand the ways that specific ge...
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Article
Breeding for sustainable oilseed crop yield and quality in a changing climate
As the effects of climate change continue to alter crop-growing conditions year-to-year on both prime and marginal agricultural landscapes, we must consider the effects not only on yield but also on quality. T...
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Article
Open AccessEvolution of pathogen response genes associated with increased disease susceptibility during adaptation to an extreme drought in a Brassica rapa plant population
Pathogens are key components in natural and agricultural plant systems. There is evidence of evolutionary changes in disease susceptibility as a consequence of climate change, but we know little about the unde...
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Article
Genetic loci underlying quantitative resistance to necrotrophic pathogens Sclerotinia and Diaporthe (Phomopsis), and correlated resistance to both pathogens
We provide results rooted in quantitative genetics, which combined with knowledge of candidate gene function, helps us to better understand the resistance to two major necrotrophic pathogens of...
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Article
Mating compatibility and fertility studies in an herbaceous perennial Aster undergoing de novo domestication to enhance agroecosystems
De novo domestication has received recent attention because of the potential to produce new crop species with additional agroecosystem functions and useful products for climate-resilient agricultural systems o...
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Article
Sunflower pan-genome analysis shows that hybridization altered gene content and disease resistance
Domesticated plants and animals often display dramatic responses to selection, but the origins of the genetic diversity underlying these responses remain poorly understood. Despite domestication and improvemen...
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Article
Two contrasting laboratory methods improve Silphium integrifolium Michx. germination rate to agronomically acceptable levels
Organic dormancy, the inability to germinate under favorable conditions, is a common problem in many crop species and their wild relatives, leading to more variable emergence, plant density, and growth rates, ...
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Article
Open AccessDiversity and evolution of the repetitive genomic content in Cannabis sativa
The repetitive content of the genome, once considered to be “junk DNA”, is in fact an essential component of genomic architecture and evolution. In this study, we used the genomes of three varieties of Cannabis s...
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Article
Open AccessThe sunflower genome provides insights into oil metabolism, flowering and Asterid evolution
A high-quality reference for the sunflower genome (Helianthus annuus L.) and analysis of gene networks involved in flowering time and oil metabolism provide a basis for nutritional exploitation and analyses of ad...
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Article
Open AccessCompromised External Validity: Federally Produced Cannabis Does Not Reflect Legal Markets
As the most widely used illicit drug worldwide, and as a source of numerous under-studied pharmacologically-active compounds, a precise understanding of variability in psychological and physiological effects of C...
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Article
Open AccessThe repetitive component of the sunflower genome as shown by different procedures for assembling next generation sequencing reads
Next generation sequencing provides a powerful tool to study genome structure in species whose genomes are far from being completely sequenced. In this work we describe and compare different computational appr...
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Article
Open AccessTransposon fingerprinting using low coverage whole genome shotgun sequencing in Cacao (Theobroma cacao L.) and related species
Transposable elements (TEs) and other repetitive elements are a large and dynamically evolving part of eukaryotic genomes, especially in plants where they can account for a significant proportion of genome siz...
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Article
Complex origin of Trinitario-type Theobroma cacao (Malvaceae) from Trinidad and Tobago revealed using plastid genomics
Trinidad and Tobago has a long history of producing high-quality cacao (Theobroma cacao L.). Cacao genotypes in Trinidad and Tobago are of a highly distinctive kind, the so-called “Trinitario” cultivar group, wid...
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Protocol
Preparation of Normalized cDNA Libraries for 454 Titanium Transcriptome Sequencing
Transcriptome sequencing from cDNA libraries has been extensively and efficiently used to analyze sequence variation in protein-coding genes (Expressed Sequence Tags) in eukaryote species. Rapid advances in ne...
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Article
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