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Protocol
High-Throughput Kinase Assay Based on Surface Plasmon Resonance
We have designed a novel high-throughput (HTP) kinase assay using an array-based surface plasmon resonance (SPR) apparatus. For high flexibility and performance, the kinase assay procedure is divided into an i...
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Article
Human protein factory for converting the transcriptome into an in vitro–expressed proteome
A collection of 33,275 human Gateway entry clones and complementary in vitro protein expression methodologies are described that allow proteome-scale production of human proteins. This 'human protein factory' was...
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Article
A Novel Nuclear Export Inhibitor JBIR-02, a New Piericidin Discovered from Streptomyces sp. ML55
A new member of the piericidin family, JBIR-02, was isolated from mycelium of Streptomyces sp. ML55 together with two known piericidin derivatives, piericidin A1 and IT-143-B. The structure was determined on the ...
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Article
Open AccessA practical device for pinpoint delivery of molecules into multiple neurons in culture
We have developed a device for pinpoint delivery of chemicals, proteins, and nucleic acids into cultured cells. The principle underlying the technique is the flow of molecules from the culture medium into cell...
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Article
Complete sequencing and characterization of 21,243 full-length human cDNAs
As a base for human transcriptome and functional genomics, we created the “full-length long Japan” (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and fou...
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Article
Identification of a ≦600-kb region on human chromosome 1q42.3 inducing cellular senescence
The introduction of a human chromosome 1 via microcell-mediated chromosome transfer (MMCT) induces the cellular senescence in mouse melanoma B16-F10 cells. The senescent cells maintained still the telomerase a...
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Article
Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif
The transcription factor NF-E2, a heterodimeric protein complex composed of p45 and small Maf family proteins, is considered crucial for the regulation of erythroid gene expression and platelet formation. To f...
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Article
A new DNA profiling system for cell line identification for use in cell banks in Japan
Using the polymorphic DNA probes, ChdTC-15, ChdTC-114, pYNH24, and λTM-18, a DNA profiling system was developed that verified identities of individual cultured cell lines collected in the Japanese cell banks, ...
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Article
Restriction fragment length polymorphisms detected in N-ras-related sequences of rats and their linkage analyses
Novel restriction fragment length polymorphisms (RFLPs) in inbred rats were revealed with the human N-ras gene as probe. Three fragments hybridizing to the probe were detected by Southern blot hybridization under...
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Article
Novel restriction fragment length polymorphism of the growth hormone gene in inbred rats
A novel restriction fragment length polymorphism in inbred rats was detected by Southern blot analysis with rat growth hormone cDNA as a probe. Four alleles, characterized byPstI fragments of 1.2, 1.1, 0.9, and 0...
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Article
Novel restriction fragment length polymorphism of the growth hormone gene in inbred rats
A novel restriction fragment length polymorphism in inbred rats was detected by Southern blot analysis with rat growth hormone cDNA as a probe. Four alleles, characterized byPstI fragments of 1.2, 1.1, 0.9, and 0...
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Article
Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor
A novel v-erb-B-related gene, c-erb-B-2, which has been identified in the human genome1,2, maps to human chromosome 17 at q21 (ref. 40), and seems to encode a polypeptide with a kinase domain that is highly homol...
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Article
Nucleotide sequence of small ColE1 derivatives: Structure of the regions essential for autonomous replication and colicin E1 immunity
A small ColE1 derivative, pAO2, which replicates like the original ColE1 and confers immunity to colicin E1 on its host cell has been constructed from a quarter region of ColE1 DNA (Oka, 1978). The entire nucl...
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Article
Nucleotide sequence at the insertion sites of a kanamycin transposon
THE TRANSPOSON Tn903 carrying a gene for kanamycin resistance, is 3,100 base pairs in size and contains an inverted repeat sequence of 1,050 base pairs at both ends1–4. We report here the generation of a 9-base p...
