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Protocol
Chromatin Immunostaining of Plant Nuclei
Recent evidence has demonstrated that specific epigenetic changes are also related to plant growth and development. Immunostaining enables the detection and characterization of chromatin modification, e.g., hi...
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Article
Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy
The chromosome compaction of chromatin fibers results in the formation of the nucleosome, which consists of a DNA unit coiled around a core of histone molecules associated with linker histone. The compaction o...
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Article
Global epigenetic changes of histone modification under environmental stresses in rice root
Abiotic stresses are non-living factors with negative morphological and physiological effects on living organisms. Substantial evidence exists that gene expression changes during plant cell growth are regulate...
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Article
Molecular insights into the non-recombining nature of the spinach male-determining region
Spinach (Spinacia oleracea L.) is a dioecious plant with male heterogametic sex determination and homomorphic sex chromosomes (XY). The dioecism is utilized for producing commercial hybrid seeds, and hence unders...
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Chapter
Flowering Genes and Homeotic Floral Gene Analysis in Jatropha
Jatropha, Jatropha curcas L., is a monoecious species, which forms unisexual flowers. The unisexual flowers are produced on the same inflorescence with 10 to 30-folds higher ratio of male flowers to female flower...
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Article
Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes
To construct a high-resolution pachytene chromosome map, we used the chromosome image analyzing system version 3 and fluorescence in situ hybridization. Two ribosomal RNA genes (45S rDNA and 5S rDNA), two major t...
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Article
Chromosome maps of legumes
Legumes are of immense importance as food and feed, and for sustainable agriculture, due to their ability to fix nitrogen. Here, the chromosome maps of the legumes soybean (Glycine max), Lotus (Lotus japonicus), ...