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    Protocol

    Chromatin Immunostaining of Plant Nuclei

    Recent evidence has demonstrated that specific epigenetic changes are also related to plant growth and development. Immunostaining enables the detection and characterization of chromatin modification, e.g., hi...

    Nobuko Ohmido, Aqwin Polosoro in Plant Cytogenetics and Cytogenomics (2023)

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    Article

    Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy

    The chromosome compaction of chromatin fibers results in the formation of the nucleosome, which consists of a DNA unit coiled around a core of histone molecules associated with linker histone. The compaction o...

    Channarong Sartsanga, Rinyaporn Phengchat, Kiichi Fukui in Chromosome Research (2021)

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    Article

    Global epigenetic changes of histone modification under environmental stresses in rice root

    Abiotic stresses are non-living factors with negative morphological and physiological effects on living organisms. Substantial evidence exists that gene expression changes during plant cell growth are regulate...

    Aqwin Polosoro, Wening Enggarini, Nobuko Ohmido in Chromosome Research (2019)

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    Article

    Molecular insights into the non-recombining nature of the spinach male-determining region

    Spinach (Spinacia oleracea L.) is a dioecious plant with male heterogametic sex determination and homomorphic sex chromosomes (XY). The dioecism is utilized for producing commercial hybrid seeds, and hence unders...

    Tomohiro Kudoh, Mitsuhiko Takahashi, Takayuki Osabe in Molecular Genetics and Genomics (2018)

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    Chapter

    Flowering Genes and Homeotic Floral Gene Analysis in Jatropha

    Jatropha, Jatropha curcas L., is a monoecious species, which forms unisexual flowers. The unisexual flowers are produced on the same inflorescence with 10 to 30-folds higher ratio of male flowers to female flower...

    Nobuko Ohmido, Eri Makigano, Suguru Tsuchimoto, Kiichi Fukui in The Jatropha Genome (2017)

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    Article

    Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes

    To construct a high-resolution pachytene chromosome map, we used the chromosome image analyzing system version 3 and fluorescence in situ hybridization. Two ribosomal RNA genes (45S rDNA and 5S rDNA), two major t...

    Nobuko Ohmido, Akiko Ishimaru, Seiji Kato, Shusei Sato in Chromosome Research (2010)

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    Article

    Chromosome maps of legumes

    Legumes are of immense importance as food and feed, and for sustainable agriculture, due to their ability to fix nitrogen. Here, the chromosome maps of the legumes soybean (Glycine max), Lotus (Lotus japonicus), ...

    Nobuko Ohmido, Shusei Sato, Satoshi Tabata, Kiichi Fukui in Chromosome Research (2007)