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Article
Open AccessBlood and urine multi-omics analysis of the impact of e-va**, smoking, and cessation: from exposome to molecular responses
Cigarette smoking is a major preventable cause of morbidity and mortality. While quitting smoking is the best option, switching from cigarettes to non-combustible alternatives (NCAs) such as e-vapor products i...
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Article
Open AccessChromosome-level genome assemblies of Nicotiana tabacum, Nicotiana sylvestris, and Nicotiana tomentosiformis
The Solanaceae species Nicotiana tabacum, an economically important crop plant cultivated worldwide, is an allotetraploid species that appeared about 200,000 years ago as the result of the hybridization of diploi...
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Article
Open AccessSuppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.)
Anatabine, although being one of four major tobacco alkaloids, is never accumulated in high quantity in any of the naturally occurring species from the Nicotiana genus. Previous studies therefore focused on trans...
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Article
Open AccessResults and lessons learned from the sbv IMPROVER metagenomics diagnostics for inflammatory bowel disease challenge
A growing body of evidence links gut microbiota changes with inflammatory bowel disease (IBD), raising the potential benefit of exploiting metagenomics data for non-invasive IBD diagnostics. The sbv IMPROVER m...
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Article
Open AccessCrowdsourced benchmarking of taxonomic metagenome profilers: lessons learned from the sbv IMPROVER Microbiomics challenge
Selection of optimal computational strategies for analyzing metagenomics data is a decisive step in determining the microbial composition of a sample, and this procedure is complex because of the numerous tool...
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Article
Open AccessThe clove (Syzygium aromaticum) genome provides insights into the eugenol biosynthesis pathway
The clove (Syzygium aromaticum) is an important tropical spice crop in global trade. Evolving environmental pressures necessitate modern characterization and selection techniques that are currently inaccessible t...
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Article
Open AccessA 6-month inhalation toxicology study in Apoe−/− mice demonstrates substantially lower effects of e-vapor aerosol compared with cigarette smoke in the respiratory tract
Cigarette smoking is the major cause of chronic obstructive pulmonary disease. Considerable attention has been paid to the reduced harm potential of nicotine-containing inhalable products such as electronic ci...
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Chapter
Characterizing the Genome of Nicotiana tabacum
The tobacco plant is an important crop and model organism, and there is a widespread interest in improving its agronomical properties. Unraveling its genome is necessary for understanding and predicting its bi...
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Leaf Curing Practices Alter Gene Expression and the Chemical Constituents of Tobacco Leaves
Tobacco leaves senesce during the early curing phase, and their constituents are altered via specific enzymatic activities. Levels of the reducing sugars glucose and fructose and those of total free amino acid...
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Background and History of Tobacco Genome Resources
Nicotiana species, which share significant similarities with tomato, potato, eggplant, and pepper, originated in South America and subsequently spread to North America, Africa, and Australia. They are known to p...
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Article
Open AccessThe tobacco genome sequence and its comparison with those of tomato and potato
The allotetraploid plant Nicotiana tabacum (common tobacco) is a major crop species and a model organism, for which only very fragmented genomic sequences are currently available. Here we report high-quality draf...
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Chapter
Advances in Nicotiana Genetic and “Omics” Resources
The importance of the genus Nicotiana is both economic, as it contains species which are major cash crops, and scientific, as many of the species’ genomes are highly complex due to their evolutionary history. ...
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Article
Open AccessReference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana tomentosiformis
Nicotiana sylvestris and Nicotiana tomentosiformis are members of the Solanaceae family that includes tomato, potato, eggplant and pepper. These two Nicotiana species originate from South America and exhibit diff...
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Chapter
Genome Structure, Functional Genomics, and Proteomics in Ascidians
The marine invertebrate chordate ascidians provide us with excellent experimental systems because of their simple and tadpole body plan at the larval stage. Ascidians are key organisms for understanding the ev...
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Article
Expression of PHA polymerase genes of Pseudomonas putida in Escherichia coli and its effect on PHA formation
Poly-3-hydroxyalkanoates (PHAs) are synthesized by many bacteria as intracellular storage material. The final step in PHA biosynthesis is catalyzed by two PHA polymerases (phaC) in Pseudomonas putida. The express...
