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Article
Open AccessViral modulation of type II interferon increases T cell adhesion and virus spread
During primary varicella zoster virus (VZV) infection, infected lymphocytes drive primary viremia, causing systemic dissemination throughout the host, including the skin. This results in cytokine expression, i...
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Article
Open AccessStandardized generation of human iPSC-derived hematopoietic organoids and macrophages utilizing a benchtop bioreactor platform under fully defined conditions
There is a significant demand for intermediate-scale bioreactors in academic and industrial institutions to produce cells for various applications in drug screening and/or cell therapy. However, the applicatio...
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Article
CAR macrophages on a fast track to solid tumor therapy
Expressing chimeric antigen receptors (CARs) in macrophages has led to promising results in preclinical and clinical work. Now, induced pluripotent stem cells have been combined with a second-generation CAR to...
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Protocol
Adaptation of Human iPSC-Derived Macrophages Toward an Alveolar Macrophage-Like Phenotype Post-Intra-Pulmonary Transfer into Murine Models
Alveolar macrophages (AMs) represent crucial immune cells in the bronchioalveolar space of the . Given the important role in the host defense machinery and tissue homeostasis, AMs have been linked to a vari...
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Article
Open AccessAn evaluation of an open access iPSC training course: “How to model interstitial lung disease using patient-derived iPSCs”
Interstitial lung diseases (ILD) are a group of rare lung diseases with severe outcomes. The COST Innovator Grant aims to establish a first-of-a-kind open-access Biorepository of patient-derived induced plurip...
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Article
Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors
Macrophages derived from human induced pluripotent stem cells (iPSCs) have the potential to enable the development of cell-based therapies for numerous disease conditions. We here provide a detailed protocol f...
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Protocol
Generation of Human iPSC from Small Volume Peripheral Blood Samples
The generation of induced pluripotent stem cells (iPSCs) from patients has opened new doors to gain insights into disease pathophysiology and treatment. In particular, the generation of iPSCs from patients who...
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Chapter
Pulmonale Alveolarproteinose
Die Alveolarproteinose ist eine sehr seltene Erkrankung mit Akkumulation von Surfactant-Proteinen und Phospholipiden in den Alveolen. Die primäre autoimmune PAP ist die häufigste Form und ist mit Nachweis von ...
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Article
Open AccessFrom macrophage biology to macrophage-based cellular immunotherapies
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Article
Open AccessPulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo
Inherited deficiency of the antiprotease alpha-1 antitrypsin (AAT) is associated with liver failure and early-onset emphysema. In mice, in vivo lentiviral transduction of alveolar macrophages (AMs) has been de...
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Article
Open AccessMakrophagen in der Krankheitsentstehung und Therapie
Macrophages are unique cells of the innate immunity and can be found in various tissues (tissue resident macrophages; TRMs). Macrophages and TRMs play a pivotal role in tissue homeostasis and can contribute to...
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Article
Lentiviral gene therapy and vitamin B3 treatment enable granulocytic differentiation of G6PC3-deficient induced pluripotent stem cells
Induced pluripotent stem cells (iPSCs) from patients with genetic disorders are a valuable source for in vitro disease models, which enable drug testing and validation of gene and cell therapies. We generated ...
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Article
Open AccessBioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections
The increasing number of severe infections with multi-drug-resistant pathogens worldwide highlights the need for alternative treatment options. Given the pivotal role of phagocytes and especially alveolar macr...
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Article
Open AccessAn immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasib...
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Article
Open AccessTALEN-mediated functional correction of human iPSC-derived macrophages in context of hereditary pulmonary alveolar proteinosis
Hereditary pulmonary alveolar proteinosis (herPAP) constitutes a rare, life threatening lung disease characterized by the inability of alveolar macrophages to clear the alveolar airspaces from surfactant phosp...
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Article
Open AccessThe CpG-sites of the CBX3 ubiquitous chromatin opening element are critical structural determinants for the anti-silencing function
Suppression of therapeutic transgene expression from retroviral gene therapy vectors by epigenetic defence mechanisms represents a problem that is particularly encountered in pluripotent stem cells (PSCs) and ...
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Article
Lung surfactant metabolism: early in life, early in disease and target in cell therapy
Lung surfactant is a complex mixture of lipids and proteins lining the alveolar epithelium. At the air–liquid interface, surfactant lowers surface tension, avoiding alveolar collapse and reducing the work of b...
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Article
Open AccessGeneration of HLA-Universal iPSC-Derived Megakaryocytes and Platelets for Survival Under Refractoriness Conditions
Platelet (PLT) transfusion is indispensable to maintain homeostasis in thrombocytopenic patients. However, PLT transfusion refractoriness is a common life-threatening condition observed in multitransfused pati...
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Article
Open AccessChemoprotection of murine hematopoietic cells by combined gene transfer of cytidine deaminase (CDD) and multidrug resistance 1 gene (MDR1)
Hematologic toxicity represents a major side effect of cytotoxic chemotherapy frequently preventing adequately dosed chemotherapy application and impeding therapeutic success. Transgenic (over)expression of ch...
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Article
Pulmonary macrophage transplantation therapy
Bone-marrow transplantation is an effective cell therapy but requires myeloablation, which increases infection risk and mortality. Recent lineage-tracing studies documenting that resident macrophage population...
