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Article
Open AccessFlavescence dorée phytoplasma enters insect cells by a clathrin-mediated endocytosis allowing infection of its insect vector
To perform its propagative and circulative cycle into its insect vector, the flavescence dorée phytoplasma invades different cell types. Clathrin-mediated endocytosis is used by a wide range of bacteria to inf...
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Article
Open AccessInteractions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. ...
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Article
Minor cultures as hosts for vectors of extensive crop diseases: Does Salvia sclarea act as a pathogen and vector reservoir for lavender decline?
Stolbur is a phytoplasma disease affecting crops worldwide. The planthopper Hyalesthes obsoletus is the main natural vector of ‘Candidatus Phytoplasma solani’ responsible of stolbur. In France, lavender (Lavandul...
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Article
Open AccessDifferential expression of Spiroplasma citri surface protein genes in the plant and insect hosts
Spiroplasma citri is a cell wall-less, plant pathogenic bacteria that colonizes two distinct hosts, the leafhopper vector and the host plant. Given the absence of a cell wall, surface ...
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Article
Open AccessHeterologous expression and processing of the flavescence dorée phytoplasma variable membrane protein VmpA in Spiroplasma citri
Flavescence dorée (FD) of grapevine is a phloem bacterial disease that threatens European vineyards. The disease is associated with a non-cultivable mollicute, a phytoplasma that is transmitted by the grapevin...
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Article
Open AccessMolecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA ty**
Coxiella burnetii, the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they are often expensive or not easily standardized across laboratories. In this work, C. bur...
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Protocol
PCR-Based Detection of Coxiella burnetii from Clinical Samples
Q fever is caused by Coxiella burnetii, an organism widely found in nature and responsible for infections in arthropods, pets, domestic and wild animals, as well as humans (1,2). Conventional diagnosis of Q fever...