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Article
Copper-mediated oxidative burst in Nicotiana tabacum L. cv. Bright Yellow 2 cell suspension cultures
In cell suspension cultures of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) a rapid and concentration-dependent accumulation of H2O2 is induced by excess concentrations of copper (up to 100 μM). This specific...
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Article
Transport of ascorbate into protoplasts ofNicotiana tabacum Bright Yellow-2 cell line
The uptake of ascorbate into protoplasts isolated from aNicotiana tabacum Bright Yellow-2 (BY-2) cell suspension culture was investigated. Addition of14C-labelled ascorbate to freshly isolated protoplasts resulte...
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Article
Transport of ascorbate into plasma membrane vesicles ofPhaseolus vulgaris L.
Incubation of bean hook plasma membrane vesicles in the presence of L-[14C]ascorbate (ASC) resulted in a specific recovery of significant levels of the ligand with the vesicles. The strong decrease in radioactive...
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Article
Involvement of ascorbic acid and ab-type cytochrome in plant plasma membrane redox reactions
Higher plant plasma membranes contain ab-type cytochrome that is rapidly reduced by ascorbic acid. The affinity towards ascorbate is 0.37 mM and is very similar to that of the chromaffin granule cytochromeb 561. ...