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Open AccessDefining consensus leukemia-associated immunophenotypes for detection of minimal residual disease in acute myeloid leukemia in a multicenter setting
Flow-cytometric detection of minimal residual disease (MRD) has proven in several single-institute studies to have an independent prognostic impact. We studied whether this relatively complex approach could be...
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Article
Aberrant marker expression patterns on the CD34+CD38− stem cell compartment in acute myeloid leukemia allows to distinguish the malignant from the normal stem cell compartment both at diagnosis and in remission
Acute myeloid leukemia (AML) is generally regarded as a stem cell disease. In CD34-positive AML, the leukemic stem cell has been recognized as CD38 negative. This CD34+CD38− population survives chemotherapy an...
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Article
MRD parameters using immunophenotypic detection methods are highly reliable in predicting survival in acute myeloid leukaemia
Outgrowth of minimal residual disease (MRD) in acute myeloid leukaemia (AML) is responsible for the occurrence of relapses. MRD can be quantified by immunophenoty** on a flow cytometer using the expression o...
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Article
Minimal residual disease cells in AML patients have an apoptosis-sensitive protein profile
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Article
Minimal residual disease in acute myeloid leukemia is predicted by P-glycoprotein activity but not by multidrug resistance protein activity at diagnosis
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Article
Multiparameter flow cytometric quantification of apoptosis-related protein expression
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Article
A flow cytometric method to detect apoptosis-related protein expression in minimal residual disease in acute myeloid leukemia
Minimal residual disease (MRD) cells are thought to be responsible for the persistence and relapse of acute myeloid leukemia (AML). Flow cytometric MRD detection by the establishment of a leukemia-associated p...
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Article
High percentage of CD34-positive cells in autologous AML peripheral blood stem cell products reflects inadequate in vivo purging and low chemotherapeutic toxicity in a subgroup of patients with poor clinical outcome
In this study, a high CD34% in autologous peripheral blood stem cell (PBSC) products from 71 AML patients was associated directly with a high relapse rate (P = 0.006) and inversely with disease-free survival (P =...
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Article
Functional characterization of minimal residual disease for P-glycoprotein and multidrug resistance protein activity in acute myeloid leukemia
Relapse is common in acute myeloid leukemia (AML) due to persistence of residual leukemia cells: minimal residual disease (MRD). In 102 out of 127 patients (80%), cells at diagnosis displayed one or more leuke...
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Article
Functional detection of MDR1/P170 and MRP/P190-mediated multidrug resistance in tumour cells by flow cytometry
Multidrug resistance (MDR) in tumour cells is often caused by the overexpression of the plasma membrane drug transporter P-glycoprotein (P-gp) or the recently discovered multidrug resistance-associated protein...
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Article
Cortisol is transported by the multidrug resistance gene product P-glycoprotein
The physiology of the multidrug transporter P-glycoprotein (Pgp) is still poorly understood. We now show evidence that cell lines with a high expression of Pgp display a reduced accumulation of cortisol and an...
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Article
Prenatal diagnosis of systemic disorders of the respiratory chain in cultured amniocytes and chorionic villus fibroblasts by studying the formation of lactate and pyruvate from glucose
Formation of lactate and pyruvate from glucose was studied in cultured amniocytes and chorionic villus fibroblasts from controls, either untreated or treated with azide, an inhibitor of cytochromec oxidase, or ot...
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Article
Restoration of NADH-oxidation in complex I and complex III deficient fibroblasts by menadione
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Scientific Proceedings Second International Symposium on Cytostatic Drug Resistance
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Detection of respiratory chain dysfunction by measuring lactate and pyruvate production in cultured fibroblasts
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First prenatal diagnosis of acyl-CoA oxidase deficiency
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Article
Lactate dehydrogenase and alkaline phosphatase isoenzymes and protein-bound sialic acid in regenerating rat liver
Lactate dehydrogenase (LDH) and alkaline phosphatase (AP) isoenzyme patterns and protein-bound sialic acid content were compared between normal, regenerating rat liver 10 days after partial hepatectomy and fet...
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Article
Prostaglandin E2 and cyclic AMP in tumor and plasma of breast cancer patients
Prostaglandin E2 and cyclic AMP (cAMP) levels were measured in tumors and plasma of 78 patients with benign and malignant breast tumors. Two groups of malignant tissues were found, one with a high level of PGE2 M...
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Article
Enzyme activities in regenerating liver of rats
The activities of eight enzymes were determined in the regenerating liver of rats after partial hepatectomy. For five enzymes, i.e., aldolase, lactate dehydrogenase, alanine transaminase, 5-nucleotidase, and a...
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Article
Prostaglandin E2 and cyclic AMP levels in human breast tumors
Prostaglandin E2 (PGE2) and cyclic adenosine-3′, 5′-monophosphate (cAMP) concentrations were measured in human benign and malignant breast tumors by radioimmunoassay. Two groups were found among the malignant tis...
