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Article
CRET-based immunoassay on magnetic beads for selective and sensitive detection of Nanog antigen as a key cancer stem cell marker
A method is presented for chemiluminescence resonance energy transfer (CRET) using APTES-Fe3O4 as a highly efficient energy acceptor with strong magnetic effectiveness over extended distances, while an Au@BSA-lum...
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Article
A fluorescent sensor array based on antibiotic-stabilized metal nanoclusters for the multiplex detection of bacteria
To address the need for facile, rapid detection of pathogens in water supplies, a fluorescent sensing array platform based on antibiotic-stabilized metal nanoclusters was developed for the multiplex detection ...
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Article
Ti3C2/Ni/Sm-based electrochemical glucose sensor for sweat analysis using bipolar electrochemistry
An innovative electrochemical sensor is introduced that utilizes bipolar electrochemistry on a paper substrate for detecting glucose in sweat. The sensor employs a three-dimensional porous nanocomposite (MXene...
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Article
A dual-targeting nanobiosensor for Gender Determination applying Signal Amplification Methods and integrating Fluorometric Gold and Silver Nanoclusters
A dual-targeting nanobiosensor has been developed for the simultaneous detection of AMELX and AMELY genes based on the different fluorescence signals emitted from gold and silver nanoclusters, AuNCs and AgNCs ...
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Article
Design of novel hyper-branched dendritic boehmite/gallic acid alumoxane for methylene blue removal: Adsorption mechanism and reusability
A novel hyper-branched alumoxane with dendritic structure (Alu-Den) was prepared via a polycondensation reaction between hydroxyl groups of boehmite and carboxylic groups of gallic acid for use as an adsorbent...
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Article
Sensitive colorimetric detection of miRNA-155 via G-quadruplex DNAzyme decorated spherical nucleic acid
Rapid and sensitive detection of biomarkers enables monitoring patients’ health status and can enhance the early diagnosis of deadly diseases. In this work, we have developed a new colorimetric platform based ...
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Chapter
Nanoengineered Aptamer Assisted Strategies for the Detection of Foodborne Pathogens
This chapter focuses on the detection of pathogens in food samples using novel aptamer-based assays. Aptamers, defined as oligonucleotide sequences with high affinity toward various targets, provide a less exp...
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Article
Sensitive colorimetric aptasensor based on g-C3N4@Cu2O composites for detection of Salmonella typhimurium in food and water
A new colorimetric aptasensor equipped with a novel composite of graphitic carbon nitride (g-C3N4) nanosheets and copper oxide(I) (Cu2O) nanocrystals is presented for Salmonella typhimurium (S .typhimurium). The ...
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Article
Fluorescent Turn-on Aptasensor of Staphylococcus aureus Based on the FRET Between Green Carbon Quantum Dot and Gold Nanoparticle
This paper describes a novel strategy based on fluorescence energy transfer (FRET) with green synthesis of carbon quantum dots (CQDs) and gold nanoparticles (GNPs) for the detection of one of the most pathogen...
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Article
A fluorescence nanobiosensor for detection of Campylobacter jejuni DNA in milk based on Au/Ag bimetallic nanoclusters
We designed a rapid method for DNA sensing for detection of campylobacter jejuni based on a selective DNA probe. A sequence containing 12 cytosine nucleotides was applied as a scaffold for making Au/Ag nanocluste...
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Article
A new colorimetric assay for amylase based on starch-supported Cu/Au nanocluster peroxidase-like activity
In this paper, we present a new colorimetric technique as a novel assay for the easy and direct detection of α-amylase activity. This detection system utilizes the interaction of α-amylase with starch that is ...
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Article
Enhanced peroxidase-like activity of platinum nanoparticles decorated on nickel- and nitrogen-doped graphene nanotubes: colorimetric detection of glucose
A nanostructured catalyst is introduced that demonstrates peroxidase mimicking activity. It consists of nickel- and nitrogen-doped graphene nanotubes loaded with platinum nanoparticles. Pt-decorated Ni-doped n...
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Article
Evaluation of Versatile Peroxidase’s Activity and Conformation in the Presence of a Hydrated Urea Based Deep Eutectic Solvent
The present study was designed to analyze the advantages and limitations of using choline chloride:urea (ChCl:U) deep eutectic solvent (DES) as a cosolvent for versatile peroxidase (VP). The VP activity declin...
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Article
Colorimetric aptasensor for Campylobacter jejuni cells by exploiting the peroxidase like activity of Au@Pd nanoparticles
The authors describe a method for colorimetric determination of Campylobacter jejuni (C. jejuni) in milk samples. It is based on the interaction of a specific DNA aptamer with surface protein in the cell membrane...
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Article
A selective colorimetric and fluorescence chemosensing sensor for Cr3+ based on a rhodamine base derivative
A rhodamine-conjugated coumarin (L) was used in designing a selective fluorescence chemosensor for the determination of trace amounts of Cr3+ ions in acetonitrile–water (MeCN/H2O (90:10, %v/v) solutions. The inte...
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Article
A colorimetric assay of DNA methyltransferase activity based on peroxidase mimicking of DNA template Ag/Pt bimetallic nanoclusters
DNA methylation catalyzed by DNA methyl transferase (MTase) is a significant epigenetic process for modulating gene expression. Abnormal levels of DNA MTase enzyme have been regarded as a cancer biomarker or a...
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Article
Colorimetric and energy transfer based fluorometric turn-on method for determination of microRNA using silver nanoclusters and gold nanoparticles
The authors describe a dual (colorimetric and fluorometric) detection scheme for microRNA. The method is based on the use of fluorescent DNA-modified silver nanoclusters (DNA-AgNCs) and gold nanoparticles (AuN...
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Protocol
DNA-Templated Silver Nanoclusters for DNA Methylation Detection
DNA methylation entails the covalent addition of a methyl group to C-5 position of cytosine by a family of DNA methyltransferase enzymes and has a significant role in gene regulation. Epigenetic changes such a...
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Article
Fluorometric determination of microRNA via FRET between silver nanoclusters and CdTe quantum dots
This paper describes a CdTe quantum dot-based fluorescence resonance energy transfer (FRET) based assay for the detection of the breast cancer biomarker microRNA. The method relies on energy transfer between D...
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Article
A highly sensitive electrochemical biosensor based on AuNP-modified gold electrodes for selective determination of serum levels of crosslaps
This article explains a step-wise protocol to develop an electrochemical sensor to quantify serum levels of C-telopeptide (CTX) crosslinks also known as crosslaps in a matter of minutes and with high level of ...