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  1. No Access

    Protocol

    Multi-dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells

    Fluorescence live-cell imaging that has contributed to our understanding of cell biology is now at the frontline of studying quantitative biochemistry in a cell. Particularly, technological advancements of flu...

    Songon An, Prakash Parajuli, Erin L. Kennedy, Minjoung Kyoung in Multienzymatic Assemblies (2022)

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    Article

    Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system

    This protocol describes a single vesicle-vesicle microscopy system to study Ca2+-triggered vesicle fusion. Donor vesicles contain reconstituted synaptobrevin and synaptotagmin-1. Acceptor vesicles contain reconst...

    Minjoung Kyoung, Yunxiang Zhang, Jiajie Diao, Steven Chu… in Nature Protocols (2013)

  3. No Access

    Article

    GPCRs regulate the assembly of a multienzyme complex for purine biosynthesis

    GPCRs are known to initiate a variety of signaling pathways, but their full reach in coordinating cellular events is unknown. Live cell imaging using label-free and fluorescence assays to monitor the effects o...

    Florence Verrier, Songon An, Ann M Ferrie, Haiyan Sun… in Nature Chemical Biology (2011)