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Article
TGFβ inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy
Embryonic hematopoiesis is a complex process. Elucidating the mechanism regulating hematopoietic differentiation from pluripotent stem cells would allow us to establish a strategy to efficiently generate hemat...
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Article
Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells
Human induced pluripotent stem (iPS) cells are similar to embryonic stem (ES) cells, and can proliferate intensively and differentiate into a variety of cell types. However, the hepatic differentiation of huma...
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Article
Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling
PTEN, a negative regulator of the phosphatidylinositol-3-kinase/AKT pathway, is an important modulator of insulin signaling. To determine the metabolic function of pancreatic Pten, we generated pancreas-specific
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Article
Generation of pancreatic islet cells and progenitor cells from embryonic stem cells
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Article
Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination
Nanog is a novel transcription factor specifically expressed in mouse embryonic stem cells (mES cells). It has been reported that Nanog plays an essential role in maintaining multi-potency of ES cells. The exp...
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Article
In vitro derivation of functional insulin-producing cells from human embryonic stem cells
The capacity for self-renewal and differentiation of human embryonic stem (ES) cells makes them a potential source for generation of pancreatic beta cells for treating type I diabetes mellitus. Here, we report...
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Article
Open AccessDevelopmental stage related patterns of codon usage and genomic GC content: searching for evolutionary fingerprints with models of stem cell differentiation
The usage of synonymous codons shows considerable variation among mammalian genes. How and why this usage is non-random are fundamental biological questions and remain controversial. It is also important to ex...
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Chapter and Conference Paper
A Cerebellar Feedback Error Learning Scheme Based on Kalman Estimator for Tracing in Dynamic System
Motivated by recent physiological and anatomical evidence, a new feedback error learning scheme is proposed for tracing in motor control system. In the scheme, the model of cerebellar cortex is regarded as the...
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Chapter and Conference Paper
Cooperative Motor Learning Model for Cerebellar Control of Balance and Locomotion
A computational model in the framework of reinforcement learning, called Cooperative Motor Learning (CML) model, is proposed to realize cerebellar control of balance and locomotion. In the CML model, cerebellu...
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Article
N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence
The L protein (241 kD) of vesicular stomatitis virus (VSV) is the most important subunit of the replication complex. The existence of specific localization signal in the L protein was investigated by making re...
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Article
Sequencing and rescuing a highly virulent classical swine fever virus: Chinese strain cF114 from a full-length cDNA clone
The complete nucleotide sequence of classical swine fever virus (CSFV) strain cF114 (F114 strain propagated on PK-15 cells) was cloned by RT-PCR. The analyses of nucleotide and amino acids identity between cF1...
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Article
Fibrillarin redistributes to the spindle poles and partially colocalizes with NuMA during mitosis
Fibrillarin, a major protein in the nucleolus, is known to redistribute during mitosis from the nucleolus to the cytosol, and is related to the dynamics of post-mitotic reassembly of the nucleolus. To better u...
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Article
Nuclear localization of Sindbis virus nonstructural protein nsP2
In early infection, approximately 10% of nonstructural protein nsP2 of Sindbis virus was transported into the nuclei of virus-infected BHK-21 cells. Nuclear nsP2 was dominantly associated with nuclear matrix. ...
