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Article
One-Step Immunoassay for the Detection of SARS-CoV-2 Nucleocapsid Protein Using Screened Fv-Antibodies
Fv-antibodies against the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were screened from an Fv-antibody library, and a one-step immunoassay was performed to detect...
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Article
Open AccessSTO-CVAE: state transition-oriented conditional variational autoencoder for data augmentation in disability classification
The class imbalance problem occurs when there is an unequal distribution of classes in a dataset and is a significant issue in various artificial intelligence applications. This study focuses on the severe mul...
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Article
Surface Plasmon Resonance (SPR) Biosensor for the Detection of SARS-CoV-2 Using Autodisplyaed FV-antibodies on Outer Membrane of E. coli
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (NP) participates in viral genome packaging and abundantly produced when infected. In this work, SPR biosensor for the dete...
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Article
Trends in defect passivation technologies for perovskite-based photosensor
Perovskites are semiconductor materials with the ABX3 structure, and they possess several attractive features, such as a tunable bandgap, high photoluminescence quantum yield (PLQY), charge mobility, and carri...
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Article
Open AccessScreening of Natural Compounds for CYP11A1 Stimulation Against Cell Renal Cell Carcinoma
Renal cancer therapies are challenging owing to the extensive spreading of this cancer to other organs and its ability to pose resistance to current medications. Therefore, drugs targeting novel targets are ur...
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Article
Open AccessOne-step immunoassay based on switching peptides for analyzing ochratoxin A in wines
A one-step immunoassay is presented for the detection of ochratoxin A (OTA) using an antibody complex with switching peptides. Because the switching peptides (fluorescence-labeled) were able to bind the frame ...
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Article
Open AccessOverexpression of CYP11A1 recovers cell cycle distribution in renal cell carcinoma Caki-1
Clear cell renal carcinoma is commonly known for its metastasis propensity to outspread to other organs and is asymptomatic in the early stage. Recent studies have shown that deficiencies in CYP11A1 expression...
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Article
One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher
One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the fluorescence-labeled switching peptide dissociated from the bindi...
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Article
An approach for identifying in silico peptides against authentic metabolites: in vitro characterization of thymosin β4 metabolites
Thymosin β4 is a highly active protein that exerts multiple biological activities such as tissue repair, anti-inflammation, and cell maturation. Thymosin β4 has also been listed as a prohibited drug by the Wor...
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Article
Open AccessLaser desorption/ionization mass spectrometry of L-thyroxine (T4) using combi-matrix of α-cyano-4-hydroxycinnamic acid (CHCA) and graphene
An optimal combi-matrix for MALDI-TOF mass spectrometry was presented for the analysis of L-thyroxine (T4) in human serum. For the selection of the optimal combi-matrix, several kinds of combi-matrices were prepa...
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Article
Assessment of standardization of domestic commercial black garlic extract for S-allyl-l-cysteine and S-1-propenyl-l-cysteine
Aged garlic extract (AGE) contains a significant amount of bioactive compounds, including S-allyl-l-cysteine (SAC), which is associated with various health benefits. Among different AGE products, black garlic ext...
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Article
Open AccessQuantitative analysis of vitamin D using m/MALDI-TOF mass spectrometry based on a parylene matrix chip
Vitamin D deficiency is associated with various disorders and is diagnosed based on the concentration of 25-hydroxy vitamin D3 (25(OH)D3) in serum. The parylene matrix chip was fabricated to reduce the matrix bac...
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Article
Isolation of Antibodies Against the Spike Protein of SARS-CoV from Pig Serum for Competitive Immunoassay
Several endemic corona viruses (eCoVs) have been reported to be the most common etiologic agents for the seasonal common cold and also cause pneumonia. These eCoVs share extensive sequence homology with SARS-C...
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Article
Open AccessQuantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip
A novel method for quantifying galactose was developed to serve as a newborn screening test for galactosemia using laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a TiO2 nanowire ...
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Article
Open AccessMulti-layered proteogenomic analysis unravels cancer metastasis directed by MMP-2 and focal adhesion kinase signaling
The role of matrix metalloproteinase-2 (MMP-2) in tumor cell migration has been widely studied, however, the characteristics and effects of MMP-2 in clinical sample of metastatic colorectal cancer (CRC) remain...
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Article
Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity
Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads w...
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Article
Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies
Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of ...
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Article
Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors
Anti-coronavirusdisease-2019 (COVID-19; anti-severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the ant...
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Article
An On-chip Chemiluminescent Immunoassay for Bacterial Detection using in Situ-synthesized Cadmium Sulfide Nanowires with Passivation Layers
The passivation layers of an in situ-synthesized cadmium sulfide (CdS) nanowire (NW) photosensor were prepared for two reasons: (1) to improve the physical stability of the NW on an interdigitated electrode and (...
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Article
Open AccessrGO nanomaterial-mediated cancer targeting and photothermal therapy in a microfluidic co-culture platform
We developed the microfluidic co-culture platform to study photothermal therapy applications. We conjugated folic acid (FA) to target breast cancer cells using reduced graphene oxide (rGO)-based functional nan...
