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Article
New Directions for Understanding the Codon Redefinition Required for Selenocysteine Incorporation
The fact that selenocysteine (Sec) is delivered to the elongating ribosome by a tRNA that recognizes a UGA stop codon makes it unique and a thorn in the side of what was originally thought to be a universal ge...
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Protocol
Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profiling
Deep sequencing of ribosome protected mRNA footprints, also called ribosome profiling or Ribo-Seq, is a relatively new methodology well suited to address questions regarding the mechanisms and efficiency of pr...
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Chapter
Probing Selenoprotein Translation by Ribosome Profiling
Selenoproteins with known functions are oxidoreductase enzymes that serve roles in maintaining cellular redox balance, reproductive health, thyroid hormone metabolism, development, and immune functions. Unique...
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Article
Correction: Corrigendum: Translation from a DMD exon 5 IRES results in a functional dystrophin isoform that attenuates dystrophinopathy in humans and mice
Nat Med. 20, 992–1000 (2014); doi:10.1038/nm.3628; corrected 25 August 2014; corrected after print 13 March 2015 In the version of this article initially published, three participants of the study were not inc...
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Article
Correction: Corrigendum: Translation from a DMD exon 5 IRES results in a functional dystrophin isoform that attenuates dystrophinopathy in humans and mice
Nat. Med.; 10.1038/nm.3628; corrected 25 August 2014 In the version of this article initially published online, the nucleotide numbering of the AS (−3 to + 16), B (+17 to +40) and C (+49 to −10) targeted seque...
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Article
Translation from a DMD exon 5 IRES results in a functional dystrophin isoform that attenuates dystrophinopathy in humans and mice
A novel internal ribosomal entry site in the 5' end of the dystrophin gene allows for expression of a form of the protein that could be therapeutic for certain forms of Duchenne muscular dystrophy.
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Chapter
Selenoprotein N: Its Role in Disease
Selenoprotein N is among the newly identified selenoproteins, initially discovered in silico with no known molecular function. It has become the focus of attention because mutations in the selenoprotein N gene...
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Chapter
Molecular Mechanism of Eukaryotic Selenocysteine Incorporation
Although substantial progress has been made in determining which factors are required for eukaryotic Sec incorporation, the mechanism by which the factors are able to alter the coding potential of an mRNA at ...
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Chapter
Reprogramming the Ribosome for Selenoprotein Expression: RNA Elements and Protein Factors
Many of the benefits of the antioxidant selenium can be attributed to its incorporation into selenoenzymes as the 21st amino acid, selenocysteine. Selenocysteine incorporation occurs cotranslationally at UGA c...
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Article
Independent evolution of bitter-taste sensitivity in humans and chimpanzees
The ability to sense bitter taste is vital for detecting toxins in food. Phenylthiocarbamide (PTC) is unusual in that to us it tastes either very bitter, or almost tasteless, depending on an individual's genet...
