Trypanosomatids
Methods and Protocols
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Open AccessTwo decades taken at speed: genomics, cell biology, ecology, and evolution of protists
In dynamic scientific fields, two decades can be an eternity, with technical and conceptual advances leading to drastically changed landscapes and paradigms. Noted natural philosopher Ferris Bueller once opine...
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The Protistan Origins of Animals and Fungi
Fungi and Metazoa (animals) are two major multicellular kingdoms of life and both are positioned in the eukaryotic Opisthokonta. Within the supergroup Fungi and Metazoa fall into either side of the opisthokont...
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Open AccessGenomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri
The opportunistic pathogen Naegleria fowleri establishes infection in the human brain, killing almost invariably within 2 weeks. The amoeba performs piece-meal ingestion, or trogocytosis, of brain material causin...
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Airyscan Superresolution Microscopy to Study Trypanosomatid Cell Biology
The recent introduction by Carl Zeiss Ltd. of the Airyscan detector module for their LSM880 confocal laser-scanning microscope has enabled routine superresolution microscopy to be combined with the advantages ...
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Protocol
Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts
Cellular DNA is inherently unstable, subject to both spontaneous hydrolysis and attack by a range of exogenous and endogenous chemicals as well as physical agents such as ionizing and ultraviolet radiation. Fo...
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Open AccessTranscriptome, proteome and draft genome of Euglena gracilis
Photosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability to thrive in a range of harsh environments. E. gr...
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Open AccessGenome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling
The Amoebozoa constitute one of the primary divisions of eukaryotes, encompassing taxa of both biomedical and evolutionary importance, yet its genomic diversity remains largely unsampled. Here we present an an...
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Swimming with protists: perception, motility and flagellum assembly
Flagella and cilia are used in unicellular and multicellular eukaryotes for fast cell motility, rapid movement of material over cell surfaces, cell feeding and...
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Flagellar motility is required for the viability of the bloodstream trypanosome
There is little hope for a vaccine for African slee** sickness, and most of the drugs currently used to treat it are old, not particularly effective and difficult to use in the conditions that prevail in sub...
